Optogenetics: A Roadmap, Softcover reprint of the original 1st ed. 2018 Neuromethods Series, Vol. 133

This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts.

Preface…

Table of Contents…

Contributing Authors…

1. User’s Guide to the Selection of Viral Vectors for Optogenetic Studies

K.R. Thompson and C. Towne

2. Cell Type-Specific Targeting Strategies for Optogenetics

Ofer Yizhar and Antoine Adamantidis

3. Molecular Engineering of Channelrhodopsis for Enhanced Control over the Nervous System

André Berndt

4. Optogenetic Control of Intracellular Signaling: Class II Opsins

Erik Ellwardt and Raag D. Airan

5. Optogenetics in Stem Cell Research: Focus on the Central Nervous System

Johannes Boltze and Albrecht Stroh

6. Optogenetic Applications in the Nematode Caenorhabditis elegans

Katharina Elisabeth Fischer, Nathalie Alexandra Vladis, and karl Emanuel Busch

7. Opto

Soojin Ryu and Rodrigo J. De Marco

8. Combining Optogenetics with MEA, Depth-Resolved LFPs and Assessing the Scope of Optogenetic Network Modulation

Jeng-Wei Yang, Pierre-Hugues Prouvot, Albrecht Stroh, and Heiko J. Luhmann

9. Concepts of All-Optical Physiology

Jan Doering, Ting Fu, Isabelle Arnoux, and Albrecht Stroh

10. Two-Photon Optogenetics by Computer-Generated Holography

Eirini Papagiakoumou, Emiliano Ronzitti, I-Wen Chen, Marta Gajowa, Alexis Picot, and Valentina Emiliani

11. Optophysiology and Behavior in Rodents and Non-Human Primates

Golan Karvat and Ilka Diester

12. Employing Optogenetics in Memory Research

Limor Regev and Inbal Goshen

13. Towards Opto-Magnetic Physiology: Concepts and Pitfalls

Miriam Schwalm, Eduardo Rosales-Jubal, and Albrecht Stroh

14. Optogenetics: Lighting a Path from the Laboratory to the Clinic

Hannah K. Kim, Allyson L. Alexander, and Ivan Soltesz