NMDA Receptors, Softcover reprint of the original 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1677

This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, NMDA Receptors: Methods and Protocols is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scientists, clinicians, and industry professionals working in this field.

Preface…
Table of Contents…
Contributing Authors…

1. NMDA Receptors in the Central Nervous System
Kasper B. Hansen, Feng Yi, Riley Perszyk, Frank S. Menniti, and Stephen F. Traynelis

Part I NMDAR Subunits Expression and Mutations

2.Quantification of NMDAR Subunit Genes Expression by qRT-PCR
Emilie Pallesi-Pocachard

3. Genetic and Functional Analysis of GRIN2A in Tumor Samples
Todd D. Prickett, Jared J. Gartner, and Yardena Samuels

4. Detection of NMDARs Antibodies in Encephalitis
Matteo Gastaldi, Patrick Waters, and Angela Vincent

Part II Studying Structure-Function Relations in NMDARs

5. Magnetofection^TM of NMDA Receptor Subunits GluN1 and GluN2A Expression Vectors in Non-Neuronal Host Cells
Nadine Bruneau and Pierre Szepetowski

6. Transfection in Primary Cultured Neuronal Cells
Katie F. M. Marwick and Giles E. Hardingham

7. Selective Cell-Surface Expression of Triheteromeric NMDA Receptors
Feng Yi, Stephen F. Traynelis, and Kasper B. Hansen

8. Functional Analysis of Recombinant Channels in Host Cells using a Fast Agonist Application System
Andrei Rozov and David Jappy

9. GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imaging
C. Perrio, O. Nicole, and A. Buisson

10. Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers
Meilin Tian and Shixin Ye

Part III Analysis of NMDAR Function/Activity

11. Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutations
Rolf Sprengel, Ahmed Eltokhi, and Frank N. Single

12. Electrophysiological Investigation of NMDA Current Properties in Brain Slices
Alexandre Pons Bennaceur and Natalia Lozovaya

13. Analysis of Functional NMDA Receptors in Astrocytes
Frank Kirchhoff

14. GluNs Detection and Functions in Microglial Cells
Stephane Peineau, Vincent Degos, Catherine Verney, and Pierre Gressens

15. NMDA Receptor Activity in Circulating Red Blood Cells: Methods of Detection
Asya Makhro, Lars Kaestner, and Anna Bogdanova

16. NMDA Receptors as Voltage Sensors
Roustem Khazipov

17. Development of a Computational Approach/Model to Explore NMDA Receptors Functions
A. Florence Keller, Jean-Marie C. Bouteiller, and Theodore W. Berger

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras