Opioid Receptors, Softcover reprint of the original 1st ed. 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1230

Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.

Overview of Genetic Analysis of Human Opioid Receptors.- Computational Structural Biology of Opioid Receptors.- Analysis of Epigenetic Mechanisms Regulating Opioid Receptor Gene Transcription.- Renilla Luciferase Reporter Assay to Study 3’UTR-Driven Post-Transcriptional Regulations of OPRM1.- High-Throughput Gene Expression Profiling of Opioid-Induced Alterations in Discrete Brain Areas.- Real-Time Imaging of Mu Opioid Receptors by Total Internal Reflection Fluorescence Microscopy.- In Vivo Techniques to Investigate the Internalization Profile of Opioid Receptors.- Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET).- Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-Visual Arrestins.- Identification and Verification of Proteins Interacting with the Kappa Opioid Receptor (KOPR).- Detection of Mu Opioid Receptor (MOPR) and Its Glycosylation in Rat and Mouse Brains by Western Blot with Anti-µC, an Affinity-Purified Polyclonal Anti-MOPR Antibody.- Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues.- [³⁵S]GTPgS-Autoradiography for Studies of Opioid Receptor Functionality.- Fluorescence-Based, High-Throughput Assays for µ-Opioid Receptor Activation Using Membrane Potential-Sensitive Dye.- Analysis of Potassium and Calcium Imaging to Assay the Function of Opioid Receptors.- Electrophysiological Patch Clamp Assay to Monitor t