Neurotransmitter Transporters, 1st ed. 2016 Investigative Methods Neuromethods Series, Vol. 118

Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in the further study of this vital field.

Part I: Recent Improvements in LV Construction, Production, and Transduction

1.      Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown

Angeline De Bruyns, Ben Geilin, and David Dankort

Tobias Maetzig and Axel Schambach

3.      Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions

Nathalie Holic And David Fenard

4.      Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G

Nataša Anastasov, Ines Höfig, Sabine Mall, Angela M. Krackhardt, and Christian Thirion

Part II: New LV Targets and Applications

5.      Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors

Maike Stahlhut, Axel Schambach, and Olga S. Kustikova

6.      Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors

Jessica K. Rieger and Maria Thomas

7.      Production and Concentration of Lentivirus for Transduction of Primary Human T Cells

Alan Kennedy and Adam P. Cribbs

8.      Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer

Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, and Robert Winston

9.      The LAM-PCR Method to Sequence LV Integration Sites

Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette Deichmann, and Manfred Schmidt

10.  Conditional Rnai using the Lentiviral GLTR System

Elisabeth Pfeiffenberger and Stephan Geley

11.  Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies

Aurélien Lathuilière and Bernard L. Schneider

III. Integrase-Mutant Lvs

12.  Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line

Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin Farzaneh, and MK Azaham A Hamid

13.  Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease

Ngoc B. Lu-Nguyen, Martin Broadstock, and Rafael J. Yáñez-Muñoz

14.  Development of Lentiviral Vectors for Targeted Integration and Protein Delivery

Diana Schenkwein and Seppo Ylä-Herttuala

Part IV: Production, Detection, and Engineering of Exosomes

Florian Dreyer and Andreas Baur

16.  Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes

Carolina Coscia, Isabella Parolini, Massimo Sanchez, Mauro Biffoni, Zaira Boussadia, Cristiana Zanetti, Maria Luisa Fiani, and Massimo Sargiacomo

17.  Cardiac Myocyte Exosome Isolation

Zulfiqar A. Malik and Anne A. Knowlton

18.  Incorporation of Heterologous Proteins in Engineered Exosomes

Francesco Manfredi, Paola Di Bonito, Claudia Arenaccio, Simona Anticoli, and Maurizio Federico

19.  Exosome-Mediated Targeted Delivery of Mirnas

Shin-Ichiro Ohno and Masahiko Kuroda

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts