Ion and Molecule Transport in Lysosomes Methods in Signal Transduction Series
Coordonnateurs : Gasnier Bruno, Zhu Michael X.
Lysosomes are key subcellular organelles that regulate the cell function. Many of the essential activities of the cell are dependent on lysosomes. Dysfunction is linked to multiple diseases - storage disorders, neurodegeneration, immunological diseases and cancer. This book discusses concepts and methods used to study lysosome ion and small molecule transport. The contents will not only attract accomplished investigators in need of a broad review and synthesis of this important subject but will also appeal to young investigators and trainees needing to acquire comprehensive knowledge and technical skills working with lysosomal ion channels and small molecule transporters.
Key selling features:
Preface. Editors. Contributors. Endosomal and Lysosomal Electrophysiology. Chloride Transport across the Lysosomal Membrane. Endolysosomal Patch Clamping: Approaches to Measure Vesicular Ion Channel Activities. TRPML Subfamily of Endolysosomal Channels: Concepts and Methods. Investigating the Role of Two-Pore Channel 2. (TPC2) in Zebrafish Neuromuscular Development. Functional Study of Lysosomal Nutrient Transporters. Lysosomal Vitamin B12. Trafficking. Detection of Lysosomal Membrane Permeabilization. Rapid Isolation of Lysosomes from Cultured Cells Using a Twin Strep Tag. A Transcriptomic Analysis and shRNA Screen for Intracellular Ion Channels and Transporters Regulating Pigmentation. Index
Bruno Gasnier is CNRS Research Director, Group Leader at CNRS/Paris Descartes.
Michael Xi Zhu is currently with the Department of Integrative Biology and Pharmacology at the University of Texas Health Science Center at Houston.
Date de parution : 02-2022
15.6x23.4 cm
Date de parution : 06-2020
15.6x23.4 cm
Mots-clés :
BK Channel; NAADP; Cellular Organelle Function; Lysosome Isolation; Endocytic Pathways; Endolysosomal Membranes; Cell Death and Apoptosis; HEK293T Cell; Organelle Dysfunction; Lysosomal Membrane; Storage Diseases; Lysosomal Lumen; small molecule transporters; Methylmalonyl CoA Mutase; waste disposal; OPTI Mem Medium; cell metabolism; Alexa Fluor; lysosomes; Transient Receptor Potential Superfamily; immunological diseases; HEK293 Ft Cell; Lysosomal Ca; Conventional Patch Clamp; shRNA Screen; Lysosomal Transporters; Blocking Buffer; Patch Clamp; LMP; Sanger Sequencing Analysis; Methionine Synthase; Melanosomal Proteins; Zebrafish Embryos; Patch Pipette; HEK293 Cell