Immunotherapy of Cancer, 2010 Methods and Protocols Methods in Molecular Biology Series, Vol. 651

A continuously evolving technique, immunotherapy for the treatment of cancers now incorporates the use of immune cells infused during bone marrow transplants as well as approaches like cell and gene therapy, while stem cell-based therapies, tissue engineering, and targeting have also contributed to the latest successes in pre-clinical immunotherapy studies. In Immunotherapy of Cancer: Methods and Protocols, expert researchers in the field describe detailed procedures for trainees and experts in the area of basic, clinical science who wish to undertake their own cutting-edge immunotherapy studies. In addition to the protocols, the volume also contains two general overviews providing useful updates in each area as well as summaries of recent pre-clinical and clinical trials. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Immunotherapy of Cancer: Methods and Protocols seeks to guide scientists along the path to a further developed system of immunotherapeutic treatments in order to reduce and hopefully eradicate this terrible disease.

Hypoxic Tumors and Their Effect on Immune Cells and Cancer Therapy.- Characterization of Regulatory T Cells in Tumor Suppressive Microenvironments.- Generation of Cytotoxic T Lymphocytes for Immunotherapy of EBV-Associated Malignancies.- Acquisition, Preparation, and Functional Assessment of Human NK Cells for Adoptive Immunotherapy.- Enhanced Migration of Human Dendritic Cells Expressing Inducible CD40.- Differentiation of Human Embryonic Stem Cells into Hematopoietic Cells In Vitro.- Gene Therapy to Improve Migration of T Cells to the Tumor Site.- Gene Therapy to Improve Function of T Cells for Adoptive Immunotherapy.- Cytokine-FC Fusion Genes as Molecular Adjuvants for DNA Vaccines.- Pharmacology of Anti-CD3 Diphtheria Immunotoxin in CD3 Positive T-Cell Lymphoma Trials.- Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display.- Identification of Immunogenic Peptides of the Self-Tumor Antigen: Our Experience with Telomerase Reverse Transcriptase.- Rescue, Amplification, Purification, and PEGylation of Replication Defective First-Generation Adenoviral Vectors.- Adenovirus-Mediated Interleukin (IL)-24 Immunotherapy for Cancer.- Liposomes for Gene Transfer in Cancer Therapy.- Oncolytic HSV as a Vector in Cancer Immunotherapy.- Generation of Chimeric T-Cell Receptor Transgenes and Their Efficient Transfer in Primary Mouse T Lymphocytes.- Multi-walled Carbon Nanotube (MWCNT) Synthesis, Preparation, Labeling, and Functionalization.- Cellular Immunotherapy of Cancer.

Provides a wide range of detailed routine and state of the art techniques as well as experimental designs for cancer immunotherapy Details protocols for the use of nanotechnology and stem cells in cancer immunotherapy Covers pre-clinical models for cancer immunotherapy Includes supplementary material: sn.pub/extras