Cell Reprogramming for Immunotherapy, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2097

This volume details key protocols for developing strategies in immunotherapy.  Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Reprogramming T and NK Cells

1. T Cell Reprogramming against Cancer

Samuel G. Katz and Peter M. Rabinovich

2. Identification of Cell Surface Targets for CAR T Cell Immunotherapy

Diana C. DeLucia and John K. Lee

3. Microfluidic Approach for Highly Efficient Viral Transduction

Reginald Tran and Wilbur A. Lam

4. Single-cell Cytokine Analysis to Characterize CAR-T Cell Activation

5. Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells

Roddy S. O’Connorand Michael C. Milone

6. Engineering of Natural Killer Cells for Clinical Application

Noriko Shimasaki and Dario Campana

7. Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Arash Nanbakhsh and Subramaniam Malarkannan

8. In vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection

Nathan J Schloemer, Alex M. Abel, Monica S Thakar, and Subramaniam Malarkannan

9. Immunomodulation of NK Cell Activity

Carolina I. Domaica, Jessica M. Sierra, Norberto W. Zwirner, and Mercedes B. Fuertes

Part II: Reprogramming Diverse Immunocytes

10. An Overview of Advances in Cell-based Cancer Immunotherapies Based on the Multiple Immune-cancer Cell Interactions

Jialing Zhang, Stephan S. Späth, Sherman M. Weissman, and Samuel G. Katz

11. Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy

Douglas Hanlon, Olga Sobolev, Alessandra Ventura, Aaron Vassall, Nour Kibbi, Patrick Han, Alp Yurter, Eve Robinson, Renata Filler, Kazuki Tatsuno, Richard L. Edelson

12. Reprogramming Exosomes for Immunotherapy

Qinqin Cheng, Xiaojing Shi, and Yong Zhang

Ketki Bhise, Samaresh Sau, Rami Alzhrani, and Arun K. Iyer

14. CRISPR/Cas9 Gene Targeting in Primary Mouse Bone Marrow Derived Macrophages

Will Bailis

15. Genome-Wide CRISPRi/a Screening in an In Vitro Co-culture Assay of
Human Immune Cell with Tumor Cells

Jialing Zhang, Stephan S. Späth, and Samuel G. Katz

Robert E. Means, Sounak Ghosh Roy, and Samuel G. Katz

Part III: Monitoring Reprogrammed Immune Cells In Vivo

Amer M. Najjar

18. Functional Analysis of Human Hematopoietic Stem Cells in vivo in Humanized Mice

Yuanbin Song, Rana Gbyli, Xiaoying Fu, and Stephanie Halene

Part IV: Clinical Design for Cell Therapy

19. Monitoring Allogeneic CAR-T Cells using Flow Cytometry

Agnieszka Jozwik, Alan Dunlop, Katy Sanchez, and Reuben Benjamin

20. Key Factors in Clinical Protocols for Adoptive Cell Therapy in Melanoma

Bryden Considine and Michael E Hurwitz

21. Place of Academic GMP Facilities in Modern Cell Therapy

Alexey Bersenev and Andrew Fesnak

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts