Cell Culture Techniques (2^nd Ed., 2nd ed. 2019) Neuromethods Series, Vol. 145

Series Preface…
Preface…
Table of Contents…
Contributing Authors…

1. In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model
Emma K. Hollmann, Yajuan Shi, and Ethan S. Lippmann

2. In Vitro Techniques for Assessing Neurotoxicity using Human iPSC-Derived Neuronal 1 Models
Anke M. Tukker, Fiona M.J. Wijnolts, Aart de Groot, Richard W. Wubbolts, and Remco H.S. Westerink

3. Oxidative Stress Signatures in Human Stem Cell Derived Neurons
M. Diana Neely and Aaron B. Bowman

4. Glial Reactivity in Response to Neurotoxins: Relevance and Methods
Lindsay T. Michalovicz and James P. O’Callaghan

5. Neuron-Glia Interaction Studied with In Vitro Co-Culture 
Mancino S., Serafini MM., and Viviani B.

6. Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria
Monica Rodriguez-Silva, Kristen T. Ashourian, Anthony D. Smith, and Jeremy W. Chambers

7. Epigenetic Changes in Cultured Neurons and Astrocytes 
David P. Gavin, Xiaolu Zhang, and M. Guizzetti

8. The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation
Laura Nimtz, Jördis Klose, Stefan Masjosthusmann, Marta Barenys, and Ellen Fritsche

9. Zebrafish as a Tool to Assess Developmental Neurotoxicity 
Keturah G. Kiper and Jennifer L. Freeman

10. Rat Brain Slices: An Optimum Biological Preparation for acute Neurotoxicological Studies
Gabriela Aguilera-Portillo, Aline Colonnello-Montero, Marisol Maya López, Edgar Rangel- López, and Abel Santamaría

11. Electrophysiological Neuromethodologies
Yukun Yuan and William D. Atchinson

12. A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies
Zhen He, John Panos, James Raymick, Tetyana Konak, Li Cui, Diane B. Miller, James P. O’Callaghan, Serguei Liachenko, Merle G. Paule, and Syed Z. Imam

13. Transporter Studies: Brain Punching Technique
Cherish A. Taylor and Somshuvra Mukhopadhyay

14. MicroRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders
Lena Smirnova and Alexandra Maertens

15. Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes
Anna Bal-Price and Francesca Pistollato

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts