Neurotrophic Factors (2nd Ed., 2nd ed. 2018) Methods and Protocols Methods in Molecular Biology Series, Vol. 1727
Coordonnateur : Skaper Stephen D.
1. Neurotrophic Factors: An Overview
Stephen D. Skaper
2. Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions
Paola Tirassa, Pamela Rosso, and Angela Iannitelli
3. Culture of Rodent Cortical, Hippocampal, and Striatal Neurons
Laura Facci and Stephen D. Skaper
4. Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex, Spinal Cord, and Cerebellum
Stephen D. Skaper and Laura Facci
5. Central Nervous System Neuron-Glia Co-Culture Models and Application to Neuroprotective Agents
Stephen D. Skaper and Laura Facci
6. Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays
Christine A. Webber and Douglas W. Zochodne
7. Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening
Aviva M. Tolkovsky and Jack Brelstaff
8. Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents
Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, and Laura Facci
9. Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice
Paola Brun and Hamid I. Akbarali
10. Astrocyte/Microglia Co-Cultures as a Model to Study Neuroinflammation
Laura Facci, Massimo Barbierato, and Stephen D. Skaper
11. Micropatterned Geometries Shape Oligodendrocyte and Microglia Plasticity
Cinzia Volonté, Adele De Ninno, and Susanna Amadio
12. Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair
Massimo Barbierato, Stephen D. Skaper, and Laura Facci
13. Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia
Laura Facci and Stephen D. Skaper
14. Diolistic Labeling and Analysis of Dendritic Spines
M. Foster Olive, Armani P. Del Franco, and Cassandra D. Gipson
15. Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses
Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, and Tomoyuki Takahashi
16. Methods for Quantitative Analysis of Axonal Cargo Transport
Matias Alloatti, Luciana Bruno, and Tomas L. Falzone
17. Neuronal Cell Morphology in Primary Cerebellar Granule Cells Using High-Content Analysis
Nicholas Radio and Steven Frank
18. Nerve Growth Factor-Induced Angiogenesis: 1. Endothelial Cell Tube Formation Assay
Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Hacham, Arnon Fluksman, Ofra Benny, Cezary Marcinkiewicz, and Peter I. Lelkes
19. Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay
Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, and Peter I. Lelkes
20. Rat Pancreatic Beta-Cell Culture
Myrian Velasco, Carlos Larqué, Carlos Manlio Díaz-García, Carmen Sanchez-Soto, and Marcia Hiriart
21. Growth and Neurotrophic Factors in Embryonic Stem Cells
Micaela Pannella, Luciana Giardino, Laura Calzà, and Mercedes Fernández
22. Site-Specific Direct Labeling of Neurotrophins and Their Receptors: From Biochemistry to Advanced Imaging Applications
Francesco Gobbo, Fulvio Bonsignore, Rosy Amodeo, Antonino Cattaneo, and Laura Marchetti
23. Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies
Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule, and Syed F. Ali
24. Preparation of Rat Hippocampal Organotypic Cultures and Application to Study Amyloid β-Peptide Toxicity
Maria Rosanna Bronzuoli, Roberta Facchinetti, and Caterina Scuderi
25. An Animal Model of Alzheimer Disease Based on the Intrahippocampal Injection of Amyloid β-Peptide (1-42)
Roberta Facchinetti, Maria Rosanna Bronzuoli, and Caterina Scuderi
26. Active Induction of Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice
Gabriella Contarini, Pietro Giusti, and Stephen D. Skaper
27. A Model of Systemic Inflammation to Study Neuroinflammation
Vincenzo Sorrenti, Pietro Giusti, and Morena Zusso
28. Spared Nerve Injury as a Long-Lasting Model of Neuropathic Pain
Serena Boccella, Francesca Guida, Enza Palazzo, Ida Marabese, Vito de Novellis, Sabatino Maione, and Livio Luongo
29. An In Vivo Compression Model of Spinal Cord Injury
Irene Paterniti, Emanuela Esposito, and Salvatore Cuzzocrea
30. A Controlled Cortical Impact Preclinical Model of Traumatic Brain Injury
Michela Campolo, Emanuela Esposito, and Salvatore Cuzzocrea
31. Middle Cerebral Artery Occlusion by an Intraluminal Suture Method
Rosalia Crupi, Rosanna Di Paola, Emanuela Esposito, and Salvatore Cuzzocrea
32. Primary Motor Neuron Culture to Promote Cellular Viability and Myelination
Jun-Kyo Francis Suh and Sujin Hyung
33. Culture of Human Primary Bone Cells and Phenotype Assessment
Paola Brun
34. Recreating Intestinal Peristalsis in the Petri Dish
Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez, and Carlos Barajas-López
35. Synthesis of Biocompatible Titanate Nanofibers for Effective Delivery of Neuroprotective Agents
Asya Ozkizilcik, Roger Williams, Z. Ryan Tian, Dafin F. Muresanu, Aruna Sharma, and Hari S. Sharma
36. Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides
Muge Yemisci, Secil Caban, Eduardo Fernandez-Megia, Yilmaz Capan, Patrick Couvreur, and Turgay Dalkara
Includes cutting-edge methods and protocols for the study of neurotrophic factors
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 458 p.
17.8x25.4 cm
Ouvrage de 458 p.
17.8x25.4 cm