Progenitor Cells, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 2029

Langue : Anglais

Coordonnateurs : Joglekar Mugdha V., Hardikar Anandwardhan A.

Couverture de l’ouvrage Progenitor Cells

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This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton?s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field.

Isolation and Molecular Characterization of Progenitor Cells from Human Umbilical Cord.- Isolation and Characterization of Extracellular Vesicles from Mesenchymal Stromal Cells.- Assessment of β-Cell Replication in Isolated Rat Islets of Langerhans.- Droplet Digital PCR for Measuring Absolute Copies of Gene Transcripts in Human Islet-Derived Progenitor Cells.- Isolation and In Vitro Culture of Human Gut Progenitor Cells.- Isolation and Characterization of Colony-Forming Progenitor Cells from Adult Pancreas.- A Novel Gene Delivery Approach Using Metal Organic Frameworks in Human Islet-Derived Progenitor Cells.- Encapsulation and Transplantation of Pancreatic Progenitor Cells.- Differentiation of Urothelium from Mouse Embryonic Stem Cells in Chemically-Defined Conditions.- Isolation and Characterization of Progenitor Cells from Human Adipose Tissue.- Identification and Analysis of Mouse Erythroid Progenitor Cells.- Single Cell Assays Using Hematopoietic Stem and Progenitor Cells.- Isolation and Characterization of Cardiac Progenitor Cells.- Mitochondrial Assays Using Cardiac Stem Cells.- Two-Dimensional Electrophoresis and Mass Spectrometry for Protein Identification.- High-Efficiency Lentiviral Gene-Modification of Primary Murine Bone-Marrow Mesenchymal Stem Cells.- Bone Marrow-Derived Progenitor Cells Mediate Immune Cell Regulation.- Flow Cytometry and Cell Sorting Using Hematopoietic Progenitor Cells.- Isolation of Epidermal Progenitor Cells from Rat Tympanic Membrane.- Measurement of Store-Operated Calcium Entry in Human Neural Cells: From Precursors to Differentiated Neurons.- Embryoid Body Differentiation of Mouse Embryonic Stem Cells to Neurectoderm and Neural Progenitors.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts