Biochemical Ecotoxicology Principles and Methods
Auteur : Gagne Francois
Biochemical Ecotoxicology: Principles and Methods presents practical approaches to biochemical ecotoxicology experiments for environmental protection and conservation.
With its methodical, stepped approach this essential reference introduces readers to current techniques for toxicity endpoint testing, suitable for laboratories of any size and budget. Each chapter presents a state-of-the-art principle, a quick and inexpensive procedure (including appropriate reagents), case studies, and demonstrations on how to analyze your results.
Generic techniques are covered, suitable for a variety of organisms, as well as high-throughput techniques like quantitative polymerase chain reactions and enzyme-linked immunoassays. Cutting-edge approaches, including gPCR arrays and lipidomic techniques, are also included, making this is an essential reference for anyone who needs to assess environmental toxicity.
1. Quantitative Assessments of Biochemical Assesments2. Tissue Preparation and Subcellular Fractionation Techniques3. Preparation and Maintenance of Live Tissues and Primary Cultures for Toxicity Studies4. Measuring Effects at the Gene Expression Level5. Metal Metabolism and Detoxication6. Oxidative Stress7. Xeno Biotransformation8. Cellular Energy Allocation9. Neuroendocrine Disruption10. Genotoxicity11. Biomarkers of Infection and Diseases12. Descriptive and Analytical Statistics in Biochemical Ecotoxicology13. Integration of Biomarkers into Indices
Toxicologists working in the environment, be it aquatic or terrestrial / Environmental risk assessors and analysts
- Practical, cost-effective approaches to assess environmental toxicity endpoints for all types of organism
- Presents theory, methods, case studies and information on how to analyze results
- State-of-the-art techniques, such as ‘omics’ approaches to toxicology
Date de parution : 07-2014
Ouvrage de 282 p.
19x23.3 cm
Thèmes de Biochemical Ecotoxicology :
Mots-clés :
Alternative testing; Analysis of variance; Antioxidant capacity; Aseptic conditions; Biomarkers; Biomarkers index; Biotransformation; Buffers; Calibration; Cell DNA variation; Centrifugation; Comet assay; Conjugation; Correlations; Cyclooxygenase; Cytochrome P450; DNA damage; DNA nitrosylation; DNA repair; Data distribution; Descriptive statistics; Detection limit; Discriminant function analysis; Dopamine; Ecotoxicogenomics; Effect concentration; Energy expenses; Energy reserves; Estrogens; Factorial analysis; Gene expression; Gene markers; Heat shock proteins; Heavy metals; Homogeneity of variance; Homogenization media; Labile zinc; Lipid peroxidation; Lipids; Lysozyme; Marker enzymes; Matrix effects; Metal concretions; Metallothioneins; Micronuclei; Mitochondria; Monoamine oxidase; Multi-xenobiotic resistance; Neural networks; Neuroendocrine disrupters; Nitric oxide; Normality; Normalization methods reference materials; Nuclear magnetic resonance; Nucleases; Observable effect concentration; Organ culture; Osmolarity; Oxidative stress; P-glycoprotein pump; PH; Polymerase chain reaction; Primary cultures; Proteases; Quantitative reverse transcriptase; RNA extraction; Reactive oxygen species; Replication; Reproducibility; Serotonin; Synaptosomes; Thresholds; Tissue grinders; Toxicity; Troubleshooting; Variation; Vitellogenin; Western blots