Tumor Immunology and Immunotherapy - Cellular Methods Part B

Tumor Immunology and Immunotherapy ? Cellular Methods Part B, Volume 632, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered include Quantitation of calreticulin exposure associated with immunogenic cell death, Side-by-side comparisons of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death, Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry, Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies, Methods to assess DC-dependent priming of T cell responses by dying cells, and more.

1. Quantitation of calreticulin exposure associated with immunogenic cell death Peng Liu, Liwei Zhao, Oliver Kepp and Guido Kroemer 2. Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death Lenka Kasikova, Iva Truxova, Isabelle Cremer, Catherine Sautes-Fridman, Oliver Kepp, Guido Kroemer, Radek Spisek and Jitka Fucikova 3. Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry Giulia Cerrato, Peng Liu, Isabelle Martins, Oliver Kepp and Guido Kroemer 4. Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies Martina Musella, Gwenola Manic, Claudia Galassi, Ilio Vitale and Antonella Sistigu 5. Methods to assess DC-dependent priming of T cell responses by dying cells Anne-Marie Chalupova, Sarka Vosahlikova, Daniela Rozkova, Klara Sochorova, Lenka Palova-Jelinkova, Jirina Bartunkova, Radek Spisek and Jitka Fucikova 6. Isolation of mononuclear phagocytes from the mouse gut Pailin Chiaranunt, Kyle Burrows, Louis Ngai and Arthur Mortha 7. Generation of murine bone marrow-derived macrophages and using tumor coculture assays to mimic tumor microenvironment Esra Güç, Demi Brownlie, Carolina Rodriguez Tirado, Takanori Kitamura and Jeffrey W. Pollard 8. Methodsfor macrophage differentiation and in vitro generation of human tumor associated-like macrophages Esther Heideveld, Marta Horcas-Lopez, Martha Lopez-Yrigoyen, Lesley M. Forrester, Luca Cassetta and Jeffrey W. Pollard 9. In vitro assessment of cancer cell-induced polarization of macrophages Danique E.M. Duits, Max D. Wellenstein and Karin E. de Visser 10. Protocol to assess the suppression of T-cell proliferation by human MDSC Annika M. Bruger, Christophe Vanhaver, Kirsten Bruderek, Giada Amodio, Ece Tavukçuoglu, Günes Esendagli, Silvia Gregori, Sven Brandau and Pierre van der Bruggen 11. Detection and functional evaluation of arginase-1 isolated from human PMNs and murine MDSC Stefania Canè and Vincenzo Bronte 12. Functional assay to assess T-cell inhibitory properties of myeloid derived suppressor cells (MDSCs) isolated from the tumor microenvironment of murine glioma models Mahmoud S. Alghamri, Neha Kamran, Padma Kadiyala, Pedro R. Lowenstein and Maria G. Castro 13. FACS isolation and analysis of human circulating and tumor neutrophils Agnieszka Swierczak and Jeffrey W. Pollard 14. Flow cytometry-based isolation of tumor-associated regulatory T cells and assessment of their suppressive potential Kevin Kos, Martijn van Baalen, Denize A. Meijer and Karin E. de Visser 15. Assessment of lipid load in tumor-infiltrating Tregs by flow cytometry Ilenia Pacella, Alfonso Grimaldi and Silvia Piconese 16. Preparation of single cells from tumors for single-cell RNA sequencing Yonina Bykov, Sarah H. Kim and Dmitriy Zamarin 17. Characterization of the tumor immune infiltrate by multiparametric flow cytometry and unbiased high-dimensional data analysis Romina E. Araya and Romina S. Goldszmid 18. Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF) Pauline Maby, Aurélien Corneau and Jérôme Galon 19. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system Maria Belen Garcia-Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Núñez, David Altshuler, Pedro Ricardo Lowenstein and Maria Graciela Castro 20. Analysis of the immune status from peripheral whole blood with a single-tube multicolor flow cytometry assay Anna-Jasmina Donaubauer, Ina Becker, Paul F. Rühle, Rainer Fietkau, Udo S. Gaipl and Benjamin Frey 21. Ex vivo assessment of in vivo DC-targeted antibodies in pre-clinical models Qingrong Huang and Niroshana Anandasabapathy 22. Flow cytometry-based assessment of direct-targeting anti-cancer antibody immune effector functions Michelle L. Miller and Olivera J. Finn 23. Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor models Donatella Negri, Paola Sestili, Martina Borghi, Maria Ciccolella and Laura Bracci 24. High-throughput analysis of cell-cell crosstalk in ad hoc designed microfluidic chips for oncoimmunology applications Arianna Mencattini, Adele De Ninno, Jacopo Mancini, Luca Businaro, Eugenio Martinelli, Giovanna Schiavoni and Fabrizio Mattei 25. Generation of affinity ranged antigen-expressing tumor cell lines Amaia Martinez-Usatorre and Pedro Romero 26. Recombinant fusion proteins for targeting dendritic cell subsets in therapeutic cancer vaccine Stéphanie Corgnac, Natalia K. Botelho, Alena Donda and Pedro Romero

• Contains content written by authorities in the field
• Provides a comprehensive view on the topics covered
• Includes a high level of detail