Proteomics, 2009 Methods and Protocols Methods in Molecular Biology Series, Vol. 564

Langue : Anglais

Coordonnateurs : Reinders Jörg, Sickmann Albert

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Since the development of the concept of ?proteomics?, great progress has granted deeper insight into the cellular network, but the enormous range of protein abundance, dynamics, and interactions leaves many further challenges in the field. In Proteomics: Methods and Protocols, experts in proteomic research have contributed detailed techniques, which present an overview of the contemporary challenges and possibilities in the various areas of proteomics, as well as cutting-edge protocols that provide concrete examples for successful proteome analysis. Written in the clear and concise Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Proteomics: Methods and Protocols will serve as a valuable reference book for laboratories around the world and raise interest in the complex and vital field of proteomics research.

to Proteomics.- Electrophorestic Separations.- High-Resolution Two-Dimensional Electrophoresis.- Non-classical 2-D Electrophoresis.- Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis.- Mass Spectrometry and Tandem Mass Spectrometry Applications.- MALDI MS.- Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery.- A Newcomer’s Guide to Nano-Liquid-Chromatography of Peptides.- Multidimensional Protein Identification Technology.- Characterization of Platelet Proteins Using Peptide Centric Proteomics.- Identification of the Molecular Composition of the 20S Proteasome of Mouse Intestine by High-Resolution Mass Spectrometric Proteome Analysis.- Quantitative Proteomics.- Liquid Chromatography–Mass Spectrometry-Based Quantitative Proteomics.- iTRAQ-Labeling of In-Gel Digested Proteins for Relative Quantification.- Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis.- Interpretation of Mass Spectrometry Data.- Algorithms and Databases.- Shotgun Protein Identification and Quantification by Mass Spectrometry.- Analysis of Protein Modifications.- Proteomics Identification of Oxidatively Modified Proteins in Bra.- Isotope-Labeling and Affinity Enrichment of Phosphopeptides for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry.- Subcellular Proteomics.- VII Subcellular Proteomics Organelle Proteomics: Reduction of Sample Complexity by Enzymatic In-Gel Selection of Native Proteins.- Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems.- Enrichment and Preparation of Plasma Membrane Proteins from Arabidopsis thaliana for Global Proteomic Analysis Using Liquid Chromatography-TandemMass Spectrometry.- Analysis of Protein Interactions.- Tandem Affinity Purification of Protein Complexes from Mammalian Cells by the Strep/FLAG (SF)-TAP Tag.- Sequential Peptide Affinity Purification System for the Systematic Isolation and Identification of Protein Complexes from Escherichia coli.- Bioinformatical Approaches to Detect and Analyze Protein Interactions.

Comprised of both overview articles and detailed protocols for core proteomics techniques Covers the hot topics in proteomics research, such as relative and absolute protein quantification, organellar and membrane studies as well as PTM and protein interaction analysis Contains method descriptions for both beginners and advanced proteomics researchers on electrophoresis, chromatography, and mass spectrometry Provides guidance on proteomics methods from sample preparation to bioinformatical data interpretation written by experts in the respective fields