Programmed Necrosis, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1857
This volume explores the latest techniques and approaches used to study this emerging form of cell death. The chapters in this book cover topics such as distinguishing necroptosis from apoptosis; detecting MLKL oligomerization during programmed necrosis; analyzing the RIPK3 necrosome complex; using RIPK1 kinase small molecule inhibitors in studying necroptosis; monitoring RIPK1 phosphorylation in the TNFR1 signaling complex; and assessing in vivo kidney cell death in glomerular injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Programmed Necrosis: Methods and Protocols is a valuable resource for technicians, graduate students, postdoctoral fellows, and other scientists who are pursuing studies in this field.
Preface…
Table of Contents…
Contributing Authors…
1. Tools in the Art of Studying Necroptosis
Adrian T. Ting
2. Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules
David Mark Moquin and Francis Ka-Ming Chan
3. Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis
Danish Saleh and Alexei Degterev
4. Distinguishing Necroptosis from Apoptosis
Inbar Shlomovitz, Sefi Zargarian, Ziv Erlich, Liat Edry-Botzer, and Motti Gerlic
5. Methods for Studying TNF-Mediated Necroptosis in Cultured Cells
Zikou Liu, John Silke, and Joanne M. Hildebrand
6. Analysis of Necroptosis in Bone Marrow-Derived Macrophages
Diana Legarda and Adrian T. Ting
7. Generation and Use of Chimeric RIP Kinase Molecules to Study NecroptosisDiego A. Rodriguez and Douglas R. Green
8. Detection of MLKL Oligomerization during Programmed Necrosis
Zhenyu Cai and Zheng-Gang Liu
9. Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation
Roshan J. Thapa, Shoko Nogusa, and Siddharth Balachandran
10. Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) during Necroptosis
Rosalind L. Ang and Adrian T. Ting
11. Use of RIP1 Kinase Small Molecule Inhibitors in Studying Necroptosis
Allison M. Beal, John Bertin, and Michael A. Reilly
12. Analyzing Necroptosis using a RIPK1 Kinase Inactive Mouse Model of TNF Shock
Matija Zelic and Michelle A. Kelliher
13. Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury
Wulf Tonnus, Moath Al-Mekhlafi, Christian Hugo, and Andreas Linkermann
14. Assessment of In Vivo Kidney Cell Death: Glomerular Injury
Wulf Tonnus, Moath Al-Mekhlafi, Florian Gembardt, Christian Hugo, and Andreas Linkermann
15. Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling
Joshua D. Webster, Margaret Solon, Susan Haller, and Kim Newton
16. Characterization of the TNFR1-SC using ‘Modified Tandem Affinity Purification’ in Conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS)Matthias Reichert, Amandeep Bhamra, Sebastian Kupka, and Henning Walczak
17. Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex
Dario Priem, Yves Dondelinger, and Mathieu JM Bertrand
18. Analysis of CYLD Proteolysis by Caspase-8 in Bone Marrow-Derived MacrophagesDiana Legarda and Adrian T. Ting
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 191 p.
17.8x25.4 cm
Date de parution : 08-2018
Ouvrage de 191 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 105,49 €
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