Molecular & biochemical toxicology (4th Ed.)
Auteur : Smart Robert C.
Contributors.
1. Molecular and Biochemical Toxicology: Definition and Scope (Ernest Hodgson and Robert C. Smart).
1.1 Introduction.
1.2 Toxicology.
1.3 Biochemical Toxicology.
1.4 Cellular Toxicology.
1.5 Molecular Toxicology.
1.6 Proteomics, Metabolomics.
1.7 Conclusions.
Suggested Reading.
2. Overview of Molecular Techniques in Toxicology: Genes and Transgenes (Robert C. Smart).
2.1 Applicability of Molecular Techniques to Toxicology.
2.2 Overview of the Genetic Code and Flow of Genetic Information.
2.3 Molecular Cloning.
2.4 Southern and Northern Blot Analyses.
2.5 Polymerase Chain Reaction (PCR).
2.6 Some Methods to Evaluate Gene Expression and Regulation.
2.7 Methods to Evaluate Gene Function.
3. Toxicogenomics (Marjorie F. Oleksiak).
3.1 Introduction.
3.2 A Primer of Genomics.
3.3 Tools and Approaches.
3.4 Genomes.
3.5 Functional Genomics.
3.6 Conclusions.
4. Proteomics (B. Alex Merrick).
4.1 Introduction to Proteomics.
4.2 Properties of Proteins.
4.3 Fields of Proteomic Research.
4.4 Mass Spectrometers and Protein Identification.
4.5 Proteomic Platforms.
4.6 Proteomes and Subproteomes: Expectations and Reality.
4.7 Summary.
5. Metabolomics (Nigel Deighton).
5.1 Introduction.
5.2 Methods.
5.3 Diagnostics and Functional Genomics.
5.4 Metabolomics and Toxicology.
6. Bioinformatics (Eric A. Stone and Dahlia M. Nielsen).
6.1 Introduction.
6.2 Obtaining the Genbank Record of a Known Gene.
6.3 Sequence Comparison.
6.4 Database Searching With BLAST.
6.5 Extensions to Multiple Sequences.
6.6 Genetic Mapping.
6.7 Conclusion.
7 Immunochemical Techniques in Toxicology (Gerald A. LeBlanc).
7.1 Introduction.
7.2 Definitions.
7.3 Immunogens and Antigens.
7.4 Polyclonal Antibodies.
7.5 Monoclonal Antibodies.
7.6 Immunoassays.
7.7 Conclusions.
8. Cellular Techniques (Sharon A. Meyer).
8.1 Introduction.
8.2 Cellular Studies in Intact Tissue.
8.3 Studies with Dispersed, Isolated Cells.
8.4 Monolayer Cell Culture.
8.5 Observation of Cultured Cells.
8.6 Indicators of Toxicity.
8.7 Artifacts and Confounders.
8.8 Replacement of Animal Testing with Cell Culture Models.
8.9 Conclusions.
9. Structure, Mechanism and Regulation of Cytochromes P450 (Darryl C. Zeldin and John M. Seubert).
9.1 Introduction.
9.2 Complexity of the Cytochrome P450 Gene Superfamily.
9.3 Cytochrome P450 Structure.
9.4 Mechanisms of P450 Catalysis.
9.5 Cytochrome P450 Regulation.
9.6 Transgenic Animal Models.
9.7 Reactive Oxygen Species.
9.8 Posttranslation Modification of P450s.
9.9 Summary.
10. Phase I Metabolism of Toxicants and Metabolic Interactions (Ernest Hodgson, Parikshit C. Das, Taehyeon M. Cho and Randy L. Rose).
10.1 Introduction.
10.2 Microsomal Monooxygenations: General Background.
10.3 Nonmicrosomal Oxidations.
10.4 Cooxidation by Prostaglandin Synthetase.
10.5 Reduction Reactions.
10.6...
Date de parution : 08-2008
Ouvrage de 958 p.