Hematopoietic Stem Cells, 1st ed. 2023 Methods and Protocols Methods in Molecular Biology Series, Vol. 2567

Part I: Overview

1. The Factory of Blood Production: Hematopoietic Stem Cells

            Jonathan Hoggatt and Louis M. Pelus

Part II: Molecular Modification and Evaluation

2. Detection of DNA Damage in Hematopoietic Stem Cells

            Saipriya Ayyar and Isabel Beerman

3. Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation

            Liqiong Liu

4. CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells

            Reza Shahbazi, Patricia Lipson, Karthikeya S.V. Gottimukkala, Daniel D. Lane, and Jennifer E. Adair

5. Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

            Chuanfeng Wu, So Gun Hong, Aylin Bonifacino, and Cynthia E. Dunbar

Part III: Cellular Isolation and Characterization

6. Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

            Stefan Radtke and Hans-Peter Kiem

7. Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells

            Diego A. López and Anna E. Beaudin

8. Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype

            Safa F. Mohamad and Maegan L. Capitano

9. Hematopoietic Stem Cell Identification Post-Radiation

            Andrea M. Patterson, Christie M. Orschell, and Louis M. Pelus

Part IV: The Hematopoietic Niche and Accessory Cells

10. Intravital Microscopy for Hematopoietic Studies

            Myriam Haltalli and Cristina Lo Celso

11. Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology

            Christa Haase, Dmitry Richter, and Charles P. Lin

12. MSC and HSPC Co-Culture: Mimicking Ex Vivo Bone Marrow Niche

            Pratibha Singh

13. Isolation of Thymus Stromal Cells from Human and Murine Tissue

            Karin Gustafsson and David T. Scadden

Part V: Transplantation and In Vivo Models

14. Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantation

            Scott H. Cooper, Maegan L. Capitano, and Hal E. Broxmeyer

15. Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish

            Ellen Fraint, Peng Lv, Feng Liu, Teresa V. Bowman, and Owen J. Tamplin

16. Establishing a Murine Model of the Hematopoietic Acute Radiation Syndrome

            P. Artur Plett, Louis M. Pelus, and Christie M. Orschell

17. Purinergic Signaling and Its Role in Mobilization of Bone Marrow Stem Cells

            Malwina Suszynska, Mateusz Adamiak, Arjun Thapa, Monika Cymer, Janina Ratajczak, Magdalena Kucia, and Mariusz Z. Ratajczak

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts