Glycan Microarrays, 1st ed. 2022 Methods and Protocols Methods in Molecular Biology Series, Vol. 2460

This volume encompasses short targeted reviews and methods featuring the construction and use of glycan microarray platforms. Chapters guider readers through glycan microarrays comprised of synthetic glycans, natural biomolecules, bacteria, neoglycoproteins,  various applications and progress of these platforms. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform.

Part I: Glycan microarray platforms

1. Neoglycoprotein and glycoprotein printing on a hydrogel functionalized microarray surface and incubation with labeled lectins

Jared Q. Gerlach, Marie Le Berre, and Michelle Kilcoyne

2. Evaluation of glycan-binding specificity by glycoconjugate microarray with an evanescent-field fluorescence detection system

Lalhaba Oinam and Hiroaki Tateno

3. Multiplex glycan bead array (MGBA) for high throughput and high content analyses of glycan binding proteins including natural anti-glycan antibodies

Sharad Purohit and Jin-Xiong She

4. Nanocube-based fluidic glycan array

Hung-Jen Wu, Akshi Singla, and Joshua D. Weatherston

Part II: Glycan microarray data extraction, processing and use for measurements

5. General strategies for glycan microarray data processing and analysis

6. Calculating half maximal inhibitory concentration (IC₅₀) values from glycomics microarray data using GraphPad Prism

            Marie Le Berre, Jared Q. Gerlach, Iwona Dziembała, and Michelle Kilcoyne

Part III: Complex natural and synthetic glycoconjugate microarrays

7. Synthetic plant glycan microarrays as tools for plant biology

Colin Ruprecht and Fabian Pfrengle

8. Mucin purification and printing natural mucin microarrays

9. Bacterial microarrays for examining bacterial glycosignatures and recognition by host lectins

María Asunción Campanero-Rhodes, and Dolores Solís

Chiaki Nagai-Okatani, Xia Zou, Atsushi Matsuda, Yoko Itakura, Masashi Toyoda, Yan Zhang, and Atsushi Kuno

Part IV: Applications for glycomics microarrays

Susan K. Halstead, Dawn Gourlay, and Hugh J. Willison

12. Glycan microarrays containing synthetic Streptococcus pneumoniae CPS fragments and their application to vaccine development

Paulina Kaplonek and Peter Seeberger

13. Lectin-based protein microarray for the glycan analysis of colorectal cancer biomarkers: the insulin-like growth factor system

Dragana Robajac, Martina Križáková, Miloš Šunderić, Goran Miljuš, Peter Gemeiner, Olgica Nedić, and Jaroslav Katrlík

14. Bacterial staining and profiling for glycan interactions on glycan microarrays for t-test calculation

15. Preparation and fluorescent labelling of cell-derived micelles and profiling on glycan microarrays

Marie Le Berre, Jared Q. Gerlach, and Michelle Kilcoyne

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts