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Germ Cell Development, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2770

Langue : Anglais

Coordonnateurs : Barchi Marco, De Felici Massimo

This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Germ Cell Development: Methods and Protocols provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention.

Part I: Isolation and Purification of Primordial (and) Germ Cells

 

1. Isolation and Purification of Viable PGCs from Mouse Embryos

            Massimo De Felici

 

2. Purification and Transfection Methods of Chicken Primordial Germ Cells

            Luiza Chojnacka-Puchta and Dorota Sawicka

 

3. Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells

            Janmejay Hingu, Guillermo Galdon, Nicholas A. Deebel, and Hooman Sadri-Ardekani

 

4. Purification by STA-PUT Technique of Male Germ Cells from Single Mouse and RNA-Extraction for Transcriptomic Analysis

            Chiara Naro, Claudio Sette, and Raffaele Geremia

 

5. Isolation of Mouse Germ Cells by FACS Using Hoechst 33342 and SYTO16 Double Staining

            Mark E. Gill, Hubertus Kohler, and Antoine H.F.M. Peters

 

6. Isolation and In Vitro Culture of Germ Cells and Sertoli Cells from Human Fetal Testis

            Celine M. Roelse, Arend W. Overeem, Yolanda W. Chang, Meriam Boubakri, and Susana M. Chuva de Sousa Lopes

 

Part II: Establishing In Vitro Systems for Studying Primordial (and) Germ Cells

 

7. Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression

            Naoko Irie, Toshihiro Kobayashi, and M. Azim Surani

 

8. Induction of Primordial Germ Cell-Like cells from Rat Pluripotent Stem Cells

            Mami Oikawa, Masumi Hirabayashi, and Toshihiro Kobayashi

 

9. Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions

            Xiaoyu Zhang and Ning Wang

 

10. Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cells

            Mariella Obermeier, Vera Rogiers, Tamara Vanhaecke, and Yoni Baert

 

11. Mouse In Vitro Spermatogenesis on 3D Bioprinted Scaffolds

            Guillaume Richer, Tamara Vanhaecke, Vera Rogiers, Ellen Goossens, and Yoni Baert

 

12. Histological and Cytological Techniques to Study Perinatal Mouse Ovaries and Oocytes

            Nikoleta Nikou, Maria López Panadés, and Ignasi Roig

 

13. Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model

            Pritha Dey, Noemi Monferini, Ludovica Donadini, Valentina Lodde, Federica Franciosi, and Alberto Maria Luciano

 

Part III: Somatic Reprogramming to Studying Human Aneuploidy Features

 

14. Generation of iPSC Cell Lines from Patients with Sex Chromosome Aneuploidies

            Veronica Astro and Antonio Adamo

 

Part IV: Informatic Tools for Studying Germ Cells Development and Recombination

 

15. Data Analysis Pipeline for scRNA-Seq Experiments to Study Early Oogenesis

            Wei Ge, Teng Zhang, Yang Zhou, and Wei Shen

 

16. Mapping Meiotic DNA Breaks: Two Fully-Automated Pipelines to Analyze Single-Strand DNA Sequencing Data, hotSSDS and hotSSDSextra

            Pauline Auffret, Bernard de Massy, and Julie A.J. Clément

 

17. “MeiQuant”: An Integrated Tool for Analyzing Meiotic Prophase I Spread Images

            Julien Cau, Laurine Dal Toe, Akbar Zainu, Frédéric Baudat, and Thomas Robert

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Date de parution :

Ouvrage de 288 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

189,89 €

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