Embryo Models In Vitro, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2767

1. Generation of Human Blastoids from Naïve Pluripotent Stem Cells

            Yulei Wei, Kun Liu, Carlos A. Pinzon-Arteaga, Deirdre Logsdon, Leqian Yu, Ye Yuan, and Jun Wu

2. Human Pre-Gastrulation Embryo Culture in 3D Condition

            Lifeng Xiang, Yu Yin, Gaohui Shi, and Tianqing Li

3. Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System

            Yu-Xin Luo and Yang Yu

4. Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models

            Hao Wu, Yue Wang, and Hongmei Wang

5. Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology

            Hao Wu, Yue Wang, and Hongmei Wang

6. Endometrial Assembloids to Model Human Embryo Implantation In Vitro

            Thomas M. Rawlings, Maria Tryfonos, Komal Makwana, Deborah M. Taylor, Jan J. Brosens, and Emma S. Lucas

7. Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (µPASE)

            Yi Zheng

8. Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells

            Rowan M. Karvas and Thorold W. Theunissen

9. Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells

            Amitesh Panda, Thi Xuan Ai Pham, Sherif Khodeer, and Vincent Pasque

10. Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells

            Yuchuan Miao, Margarete Diaz-Cuadros, and Olivier Pourquié

11. Generation of Stem Cell-Based Mouse Embryo-Like Structures

            Charlotte E. Handford, Pallavi Panda, Ikbal B. Mohammad Choudhury, Gianluca Amadei, and Magdalena Zernicka-Goetz

12. Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond

            Zachary T. Olmsted, Maria Belen Paredes-Espinosa, and Janet L. Paluh

13. Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts

            Sharon Arcuri, Georgia Pennarossa, Sergio Ledda, Fulvio Gandolfi, and Tiziana A.L. Brevini

14. Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast

            Asha Shaji Antony, Devika AS, and Smita Sudheer

15. Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos

            Savana Biondic, Cheng Zhao, Michael Hagemann-Jensen, Stewart J. Russell, Katherine Vandal, Jesica Canizo, Clifford L. Librach, and Sophie Petropoulos

16. Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas

            San Kit To, Bradley Balaton, and Vincent Pasque

17. Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL

            Tiam Heydari and Peter W. Zandstra

18. ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis

            Ceren Pajanoja and Laura Kerosuo

19. Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence

            Jesica Canizo, Savana Biondic, Katherine Vandal Lenghan, and Sophie Petropoulos

20. Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics

            Katherine Vandal, Savana Biondic, Jesica Canizo, and Sophie Petropoulos

21. Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos

            Jesica Canizo, Katherine Vandal, Savana Biondic, and Sophie Petropoulos

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts