Cell Tracking, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2126

This volume details methods used to track cells in the body and will serve as a reference for preclinical and clinical researchers in the fields of medicine and biomedical science.  Chapters guide readers through protocols on bioluminescence imaging, fluorescence imaging, magnetic resonance imaging (MRI), ultrasound, computed tomography, and positron emission spectroscopy.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cell Tracking: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo

Dong Han and Joseph C. Wu

2. Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations

Tej B. Shrestha and Matthew T. Basel

3. In vivo Epithelial Metabolic Imaging using a Topical Fluorescent Glucose Analog

4. Lipophilic Near Infrared Dyes for in vivo Fluorescent Cell Tracking

5. Energy-transfer Systems for in vivo Tracking

Raul Neri, Asanka Sajini Yapa, and Stefan H. Bossmann

6. Using Bioengineered Fluorescence for Selective in vivo and ex vivo Tracking of Intestinal Organoids Derived from Human Pluripotent Stem Cells

Hana Lee  and Mi-Young Son

7. Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-edited iPSC in Skeletal Muscle

Yue Jin, Yan Shen, Neal L. Weintraub, and Yaoliang Tang

8. Upconversion Fluorescent Nanoprobe for Highly-sensitive in vivo Cell Tracking

Shufang Sheng and Chao Wang

9. Magnetic Resonance Imaging of Single Cells

Madumali Kalubowilage and Stefan H. Bossmann

10. MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles

Wencheng Zhu, Ye Xu, Rongrong Jin, Changqiang Wu, and Hua Ai

11. Protease-activated Sensors for In Vivo Imaging of Cell Populations

Kayla Eschliman and Stefan H. Bossmann

12. Antibody Targeted Magnetic Nanoparticles to Track Immune Cells in Vivo

13. Stem Cell Tracking with Nanoparticle-Based Ultrasound Contrast Agents

Fang Chen and Jesse V. Jokerst

14. Gold Nanoparticles as Computer Tomography Marker for Stem Cell Tracking

Md Nafiujjaman and Taeho Kim

15. Non-Invasive Tracking of Implanted Cells: Superparamagnetic Iron Oxide Nanoparticles as a Long-Term, Multi-Modal Imaging Label

Mughil Sriramvenugopal and Christina A. Pacak

16. Comparison of the Efficacy and Sensitivity of Alternative PET Reporter Gene/PET Reporter Probe Systems that Minimizes Biological Variables

Shili Xu and Harvey R. Herschman

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts