Amyloid Proteins (3^rd Ed., Softcover reprint of the original 3rd ed. 2018) Methods and Protocols Methods in Molecular Biology Series, Vol. 1779

Preface…

Table of Contents…

Contributing Authors…

Part I: In Vitro Models and Assays

1. Preparation of Pure Populations of Amyloid-β Protein Oligomers of Defined Size 

Eric Y. Hayden, Joseph L. Conovaloff, Ashley Mason, Gal Bitan, and David B. Teplow 

2. Preparation of Well-Defined and Stable β-Barrel Pore-Forming Amyloid-β42 Oligomers 

Montserrat Serra-Batiste, Martí Ninot-Pedrosa, Eduard Puig, Sonia Ciudad, Margarida Gairí, and Natàlia Carulla

3. Unveiling Brain Aβ Heterogeneity through Targeted Proteomic Analysis

Agueda Rostagno, Thomas A. Neubert, and Jorge Ghiso

4. Preparation of α-Synuclein Amyloid Assemblies for Toxicity Experiments 

Serene W. Chen and Nunilo Cremades

5. Generation and Characterization of Stable α-Synuclein Oligomers

Leire Almandoz-Gil, Martin Ingelsson, and Joakim Bergström

6. In Vitro Analysis of α-Synuclein Amyloid Formation and Cross-Reactivity

Istvan Horvath, Sandra Rocha, and Pernilla Wittung-Stafshede

7. Preparation of Tau Oligomers after Protein Extraction from Bacteria and Brain Cortices 

Elentina K. Argyrousi, Agnieszka Staniszewski, Russell E. Nicholls, and Ottavio Arancio

8. Purification and Characterization of Low-n Tau Oligomers

 Senthilvelrajan Kaniyappan, Ram Reddy Chandupatla, and Eckhard Mandelkow

9. Preparation and Characterization of Tau Oligomer Strains

 Urmi Sengupta, Mariana Carretero-Murillo, and Rakez Kayed

10. Purification and Fibrillation of Recombinant Human Amyloid-β, Prion Protein and Tau under Native Conditions

Alexander Sandberg and Sofie Nyström

11. Preparation of Amyloidogenic  Aggregates from EF-Hand β –parvalbumin and S100 Proteins

Javier Martínez, Joana S. Cristóvão, Rosa Sánchez, Maria Gasset , and Cláudio M. Gomes

12. Kinetic Analysis of Amyloid Formation

Georg Meisl, Thomas C.T. Michaels, Sara Linse, and Tuomas P. J. Knowles

13. Mapping Amyloid Regions in Gad m 1 with Peptide Arrays 

Rosa Sánchez, Javier Martínez, Laura Montoya, Milagros Castellanos, and Maria Gasset

14. Non-Invasive Structural Analysis of Intermediate Species during Fibrillation: An Application of Small-Angle X-Ray Scattering

Annette Eva Langkilde, Fátima Herranz-Trillo, Pau Bernadó, and Bente Vestergaard

15. Analysis of Covalent Modifications of Amyloidogenic Proteins using Two-Dimensional Electrophoresis: Prion Protein and Its Sialylation

Elizaveta Katorcha and Ilia V. Baskakov

16. Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using The Real-Time Quaking-Induced-Conversion

Matthias Schmitz, Niccolò Candelise, Franc Llorens, and Inga Zerr

Part II: Cell Culture Models and Assays

17. Bacterial Amyloids

Margery L. Evans, Elizabeth Gichana, Yizhou Zhou, and Matthew R. Chapman

18. Addressing Intracellular Amyloidosis in Bacteria with RepA-WH1, a Prion-Like Protein

Laura Molina-García, Fátima Gasset-Rosa, María Moreno-del Álamo, Susana Moreno-Díaz de la Espina, and Rafael Giraldo

19. Study of Amyloids Using Yeast

Reed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, and Herman K. Edskes

20. Neurotoxic Ca2+ Signaling Induced by Amyloid-β Oligomers in Aged Hippocampal Neurons In Vitro

Lucía Núñez, María Calvo-Rodríguez, Erica Caballero, Mónica García-Durillo, and Carlos Villalobos

21. Preparation and Culturing of Human Primary Vascular Cells

Finnbogi R. Thormodsson, Ingvar H. Olafsson, and Dadi Th. Vilhjalmsson

22. Live Imaging of Pathological Tau Protein and Tau Antibodies in a Human Neuron-Like Cellular Model 

Dov B. Shamir, Yan Deng, and Einar M. Sigurdsson

23. Effects of Amyloid-β Peptide on the Biology of Human Neural Stem Cells

Adela Bernabeu-Zornoza, Raquel Coronel, María Lachgar, Charlotte Palmer, and Isabel Liste

Part III: In Vivo Models and Assays

24. Development of Mouse Monoclonal Antibodies against Human Amyloid Fibril Proteins for Diagnostic and Research Purposes

Gunilla T. Westermark, Elisabet Ihse, and Per Westermark

25. Identification and Characterization of Amyloid-β Accumulation in Synaptic Mitochondria

Shi Fang Yan, Firoz Akhter, Alexander A. Sosunov, and Shirley ShiDu Yan

26. Biochemical Properties of Pathology-Related Tau Species in Tauopathy Brains: An Extraction Protocol for Tau Oligomers and Aggregates

Naruhiko Sahara and Taeko Kimura

27. Tau Assembly into Filaments

Mar Pérez, Raquel Cuadros, and Miguel Medina

28. Quantitative Metabolomics in Alzheimer´s Disease: Technical Considerations for Improved Reproducibility

Sergio Veiga, Judith Wahrheit, Andrés Rodríguez-Martín, and Denise Sonntag 

29. Detecting Circulating MicroRNAs as Biomarkers in Alzheimer’s Disease

Aidan Kenny, Eva M. Jimenez-Mateos, Miguel Calero, Miguel Medina, and Tobias Engel

30. Luminescent Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues 

K. Peter R. Nilsson, Mikael Lindgren, and Per Hammarström

31. Characterization of Amyloid-β Plaques and Autofluorescent Lipofuscin Aggregates in Alzheimer´s Disease Brain: A Confocal Microscopy Approach

Alejandra Kun, Fernando González-Camacho, Silvia Hernández, Alexandra Moreno-García, Olga Calero, and Miguel Calero

32. In Vivo Imaging of Tauopathy in Mice

Senthilkumar Krishnaswamy, Qian Wu, Yan Lin, Wajitha J. Rajamohamedsait, Hameetha B. Rajamohamedsait, and Einar M. Sigurdsson

33. In Vivo Evaluation of Neuronal Transport in Murine Models of Neurodegeneration using Manganese-Enhanced MRI

Anne Bertrand, Maria Baron, Dung M. Hoang, Lindsay K. Hill, Sebastian L. Mendoza, Einar M. Sigurdsson, and Youssef Z. Wadghiri

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts