New Technologies for Glutamate Interaction, 1st ed. 2024 Neurons and Glia Neuromethods Series, Vol. 2780
Coordonnateur : Kukley Maria
This volume explores the latest technologies used to study the co-existence of neuronal and neuron-glia synapses in the brain. The chapters in this volume are organized into three parts. Part One covers recent advancements in the technical approaches for studying glutamatergic signalling between neurons. Part Two highlights approaches to study the functional role of astrocytes at neuronal synapses. Part Three focuses on fast signalling at neuron-glia synapses, and highlights relevant methods for investigating these unusual synapses, including slice electrophysiology and in vivo gene delivery techniques. In the Neuromethods series style, all chapters contain key advices from experts in the field that are valuable for performing successful experiments on the topic in your laboratory.
Cutting-edge and thorough, New Technologies for Glutamate Interactions: Neurons and Glia is a valuable resource for both neuronal and glial physiologists who are interested in learning about and developing new methods to further understand neuronal and neuron-glia synaptic transmission.
Series Preface…
Preface…
Table of Contents…
Contributing Authors…
Part I New Approaches to Study Glutamatergic Synapses between Neurons
1. Visualization of Glutamatergic Neurotransmission in Diverse Model Organisms with Genetically Encoded Indicators
Abhi Aggarwal, Joyce Chan, Amelia K. Waring, Adrian Negrean, Jonathan S. Marvin, Kaspar Podgorski, and Loren L. Looger2. Surface Glutamate Receptors Nanoscale Organization with Super-Resolution Microscopy (dSTORM)
Joana Ferreira and Laurent Groc
3. Ligand-Directed Chemical Labeling for Visualizing and Analyzing AMPA Receptors in Neurons
Shigeki Kiyonaka, Kyohei Soga, Kento Ojima, Hiroshi Nonaka, and Itaru Hamachi
4. Live FRET-FLIM Imaging to Study Metabotropic Signaling via the NMDA Receptor
Mehreen Manikkoth and Kim Dore
5. Quantitative Analysis of Single Glutamatergic Vesicles in the Brain
Yuanmo Wang, Ajay Pradhan, Pankaj Gupta, Hanna Karlsson-Fernberg, and Ann-Sofie Cans
6. Imaging Microtubule Network in Rodent Giant Glutamatergic Presynaptic Terminals
Laurent Guillaud
7. Photooxidation of Genetically Encoded MiniSOG-Fused VGLUT2 for Identification of Glutamatergic Synapses by Transmission and 3D Electron Microscopy
Andrew J. Flores, Anna Nilsson, Junru Hu, Mason R. Mackey, Mark H. Ellisman, Thomas S. Hnasko, and Daniela Boassa
Part II New Approaches to Study Role of Astrocytes at Neuronal Synapses
8. Chemogenetic Approaches to Study Astrocytes at Glutamatergic Synapses
Liam Nestor, Yana Van Den Herrewegen, Zuner A. Bortolotto, Dimitri De Bundel, and Ilse Smolders9. Studying the Role of Astrocytes at Synapses using Single-Cell Transcriptomics
Francisco Pestana, T. Grant Belgard, Thierry Voet, and Matthew G. Holt
10. Analysis of Synaptic Glutamate Clearance as a Possible Indicator of Synaptic Health in the Degenerating Rodent Brain
Anton Dvorzhak and Rosemarie Grantyn
11. Computational Models of Astrocyte Function at Glutamatergic Synapses
Kerstin Lenk, Audrey Denizot, Barbara Genocchi, Ippa Seppälä, Marsa Taheri, and Suhita Nadkarni
Part III Technical Approaches to Study Fast Signaling at Neuron-Glia Synapses
12. Electrophysiological Recordings of Oligodendroglia in Adult Mouse Brain Slices
Coram Guevara, Rodrigo Varas, María Cecilia Angulo, and Fernando C. Ortiz13. Synaptic Integration at Neuron-OPC Synapses
Wenjing Sun
14. Mapping Synaptic Inputs to Oligodendroglial Cells using In Vivo Monosynaptic Viral Tracing
Belgin Yalçın and Michelle Monje
15. In Vivo Viral Gene Delivery to Manipulate Functional Properties of AMPA Receptors in Oligodendrocyte Lineage Cells
Ting-Jiun Chen, Bartosz Kula, and Maria Kukley
16. Studying Synaptic Integration of Glioma Cells into Neural Circuits
Kiarash Shamardani, Kathryn R. Taylor, Tara Barron, and Michelle Monje
Subject Index List…
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 04-2024
Ouvrage de 371 p.
17.8x25.4 cm