Hypoxia, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2755
Coordonnateur : Gilkes Daniele M.
This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new protocols to characterize hypoxia in tumors with high spatial resolution and novel therapeutic approaches that consider the complex microenvironments of solid tumors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Hypoxia: Methods and Protocols is a valuable tool to help cancer researchers and other scientists detect hypoxia in cancer and other disease pathologies.
Preface…
Table of Contents…
Contributing Authors…
Part I Fluorescent Reporter Methods for Hypoxia Detection
1. Genetically Encoded Reporters to Monitor Hypoxia
2. Detection of Hypoxia in 2D and 3D Cell Culture Systems using Genetically Encoded Fluorescent Hypoxia Sensors
3. Mapping the Fate of Hypoxic Cells using an Irreversible Fluorescent Switch
4. Application of a Specific and Sensitive NQO1 Turn-On Near-Infrared Fluorescence Probe for Live Cancer Cell and Xenografted Tumor Imaging in Nude Mice
5. A Luciferase Reporter Assay to Detect Cellular Hypoxia In Vitro
6. Oxygen Assessment in Tumors In Vivo using Phosphorescence Lifetime Imaging Microscopy
Anastasia D. Komarova, Vladislav I. Shcheslavskiy, Anton A. Plekhanov, Marina A. Sirotkina, Leonid N. Bochkarev, and Marina V. Shirmanova
7. Radionuclide Reporter Imaging to Visualize Tumor Hypoxia Ex Vivo and In Vivo
Masayuki Inubushi, Yasuto Takeuchi, and Yoshimasa Kitagawa
8. Measuring Pericellular Oxygen Tension for In Vitro Cell Culture
Zachary J. Rogers, Khushbu Bhatt, and Sidi A. Bencherif
9. A Review of Hypoxia Imaging Using 18F-Fluoromisonidazole Positron Emission Tomography
Kenji Hirata, Shiro Watanabe, Yoshimasa Kitagawa, and Kohsuke Kudo
10. Photoacoustic Lifetime Imaging of Hypoxia
Qi Shao and Joshua G. Punnoose
11. Staining Hypoxic Areas in Frozen and FFPE Tissue Sections with Hypoxyprobe™
Harsh H. Oza, Esther Ng, and Daniele M. Gilkes
Part III Methods to Detect Hypoxia Inducible Factors and their Downstream Targets
12. Multiplex Immunofluorescence Staining Protocol for Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formalin-Fixed Paraffin-Embedded Samples
Harsh H. Oza and Daniele M. Gilkes
13. Detecting Hypoxia-Inducible Factor Levels and Activity
Maimon E. Hubbi
Part IV Methods to Detect Hypoxia-Induced Changes in Cell Metabolism
14. Metabolomic Investigations into Hypoxia-Mediated Metabolic Reprogramming of Pancreatic Cancer Cells
Vira Chumak, Girish H. Rajacharya, and Pankaj K. Singh
15. Measurement of Metabolic Alteration in Immune Cells under Hypoxia
Yuki Fujii and Kamiya Mehla
Part V Methods to Simulate Hypoxia, Evaluate Oxygen Consumption Rates, and Measure FIH Activity Levels
16. Evaluation of Oxygen Consumption Rates In Situ
Anqi Li, Yuan Qin, Ying Zhang, Xiaoqun Zhen, and Guohua Gong
17. Cleanroom-Free Microfluidic Device for Natural Induction of Hypoxia in 2-D and 3-D Tumor Models
Jeong Min Oh and Keyue Shen
18. Oxomer- and Reporter Gene-Based Analysis of FIH Activity in Cells
Subject Index List…
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 02-2024
Ouvrage de 266 p.
17.8x25.4 cm
