Borrelia burgdorferi, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1690

This volume details protocols that broadly cover many aspects of basic and translational research on Borrelia burgdorferi. Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, animal infection, pathogenesis and host responses, and vaccines. These essential protocols incorporate the most recent, practical, and innovative research tools  aiding new and experienced researchers in their studies involving the biology, pathogenesis, and prevention of B. burgdorferi infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Borrelia burgdorferi: Methods and Protocols aims to ensure successful results in the further study of this vital field of biomedical research.

1. Genotyping Strains of Lyme Disease Agents Directly From Ticks, Blood, or Tissue

Alan G. Barbour and Vanessa J. Cook

2. Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato using Molecular Biological Methods

Gabriele Margos, Isabell Notter, and Volker Fingerle

3. Generation of Mammalian Host-adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats

Melissa J. Caimano

4. Co-immunoprecipitation for Identifying Protein-protein Interactions in Borrelia Burgdorferi

Henna Iqbal, Darrin R. Akins, and Melisha R. Kenedy

5. Characterization of Borreliaburgdorferi Binding to Mammalian Cells and Extracellular Matrix

Yi-Pin Lin and John M. Leong

6. Analysis of Lipids and Lipid Rafts in Borrelia

Alvaro Toledo, Zhen Huang, Jorge Benach, and Erwin London

7. Trace Element AnalysisofBorrelia burgdorferi by Inductively Coupled Plasma-Sector Field Mass Spectrometry

Xin Li, Peng Wang, Anthony Lutton, and John Olesik

8. Identification and Characterization of Borrelia burgdorferiComplement-binding Proteins

Peter Kraiczy

9. Artificial Infection of Ticks with Borrelia burgdorferi using a Microinjection Method and their Detection in vivo using Quantitative PCR Targeting flaB RNA

Alexis A. Smith, Xiuli Yang, Erol Fikrig, and Utpal Pal

10. Borrelia burgdorferi Microarray Analysis

11. Borrelia burgdorferi Transcriptome Analysis by RNA-sequencing

Lybecker Mand Henderson KC

12. Selection of Borrelia burgdorferi Promoter Sequences Active during Mammalian Infection Using In Vivo Expression Technology

Philip P. Adams and Mollie W. Jewett

13. Analysis of DNA and RNA Binding Properties of Borrelia burgdorferi Regulatory Proteins

J. Seshu , Trever C. Smith II, Ying-Han Lin, S.L. Rajasekhar Karna, Christine L. Miller, and Tricia Van Laar

14. Identification of Acetylated Proteins in Borrelia burgdorferi

Youyun Yang, Alan Wolfe, and X. Frank Yang

15. Genetic Transformation and Complementation

D. Scott Samuels, Dan Drecktrah, and Laura S. Hall

16. Genome-wide Mutagenesis in Borrelia burgdorferi

Tao Lin and Lihui Gao

17. Generation of Conditional Mutants in Borrelia burgdorferi

Jacob I. Latham and Jon S. Blevins

18. Detection of Bioluminescent Borrelia burgdorferi from in vitro

Jenny A. Hyde and Jon T. Skare

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts