Characterization of Nanoparticles Intended for Drug Delivery (2nd Ed., Softcover reprint of the original 2nd ed. 2018) Methods in Molecular Biology Series, Vol. 1682
This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test sterility and endotoxin, physicochemical features, immunological effects, drug release, and in vivo efficacy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Characterization of Nanoparticles Intended for Drug Delivery, Second Edition is a valuable tool for researchers and pharmaceutical and biotechnology developers who are evaluating the clinical potential of nanomedicines in preclinical studies.>
2. Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test
3. Considerations and Some Practical Solutions to Overcome Nanoparticle Interference with LAL Assays and to Avoid Endotoxin Contamination in Nanoformulations
4. Elemental Analysis in Biological Matrices Using ICP-MS
5. PEG Quantitation Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection
6. Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis
7. Immunoelectron Microscopy for Visualization of Nanoparticles
8. Imaging of Liposomes by Transmission Electron Microscopy
9. Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Properties
10. In Vitro Assessment of Nanoparticle Effects on Blood Coagulation
11. In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells
12. Assessing NLRP3 Inflammasome Activation by Nanoparticles
13. Analysis of Complement Activation by Nanoparticles
14. Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivo
15. Analysis of Pro-Inflammatory Cytokine and Type II Interferon Induction by Nanoparticles
17. In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed Type Hypersensitivity Reactions
18. Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct
19. Improved Ultrafiltration Method to Measure Drug Release from Nanomedicines Utilizing a Stable Isotope Tracer
20. Designing an In Vivo Efficacy Study of Nanomedicines for Preclinical Tumor Growth Inhibition
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 256 p.
17.8x25.4 cm
Date de parution : 10-2017
Ouvrage de 256 p.
17.8x25.4 cm
