Cell-Based Assays Using iPSCs for Drug Development and Testing, 1st ed. 2019 Methods in Molecular Biology Series, Vol. 1994
Coordonnateurs : Mandenius Carl-Fredrik, Ross James A.
Preface…
Table of Contents…
Contributing Authors…
1. Cell-Based Assays using Differentiated Human Induced Pluripotent Cells
M Z. Cader, M. Graf, M. Burcin, C.F. Mandenius, and J.A. Ross
Part I Differentiation of iPC to Target Cells for Assays
2. Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells
Klaus Christensen, Filip Roudnicky, Mark Burcin, and Christoph Patsch
3. Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modelling
Katharina Janssen, Lamiaa Bahnassawy, Claudia Kiefer, Jürgen Korffmann, Georg C. Terstappen, Viktor Lakics, Miroslav Cik, and Peter Reinhardt
4. Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation
Anwar A. Palakkan, Jyoti Nanda, and James A. Ross
5. Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies
Caroline Halloin, Michelle Coffee, Felix Manstein, and Robert Zweigerdt
6. Improved Protocol of Cardiac Differentiation and Maturation of Pluripotent Stem Cells
Imen Jebeniani, Shunli Ding, and Michel Pucéat
7. Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors
Felix Manstein, Caroline Halloin, and Robert Zweigerdt
8. A Protocol for the One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes
Cormac Murphy, Elisabeth Feifel, Paul Jennings, Gerhard Gstraunthaler, and Anja Wilmes
9. Differentiation of hiPS Cells into Definitive Endoderm for High Throughput Screening
Teresa Bluhmki, Stefanie Traub, Eva Schruf, James Garnett, Florian Gantner, Daniel Bischoff, and Ralf Heilker
Part II High Throughput Metabolomics and Proteomic Cell-Based Assays
10. LC-MS/MS Based Metabolomics for Cell Cultures
Anna Artati, Cornelia Prehn, and Jerzy Adamski
11. Preparation of iPSCs for Targeted Proteomic Analysis
Anna Baud, Wendy E. Heywood, Daniel Little, Paul Gissen, and Kevin Mills
12. Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes
Francesca Mazzacuva, Maëlle Lorvellec, Agostino Cilibrizzi, Kevin Mills, Peter Clayton, and paul Gissen
13. Mass Spectrometry Measurement of Albumin/Alpha Fetoprotein Ratio as an Indicator of iPSC-Derived Hepatocyte Differentiation
Francesca Mazzacuva, Maëlle Lorvellec, Agostino Cilibrizzi, Kevin Mills, Wendy Heywood, Peter Clayton, and Paul Gissen
Part III High Content Imaging
14. Seeding Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well Plates
Daniel Little, Christin Luft, Oliver Pezzini-Picart, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, and Paul Gissen
15. High Content Autophagy Analysis in iPSC-Derived Neurons using Immuno-Fluorescence
Daniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, and Paul Gissen
16. High Content Analysis of Mitochondrial Function in iPSC-Derived Neurons
Daniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, and Paul Gissen
17. Evaluating the Effect of Drug Compounds on Cardiac Spheroids using the Cardiac Cell Outgrowth Assay
Jonas Christoppersson, Florian Meier, Henning Kempf, Kristin Schwanke, Michelle Coffee, Mario Beilmann, Robet Zweigerdt, and Carl-Fredrik Mandenius
Part IV Electrophysiology Assays
18. Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes using Multi-Well Microelectrode Arrays
Udo Kraushaar and Elke Guenther
19. Multielectrode Array (MEA) Based Detection of Spontaneous Network Activity in Human iPSC-Derived Cortical Neurons
Valeria Kizner, Sandra Fischer, and Maximilian Naujock
Part V Miscellaneous Cell-Based Assays
20. Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm
Valeria Kizner, Sandra Fischer, Benjamin Jähnke, and Maximilian Naujock
21. Fabrication of a Microfluidic Cell Culture Device using Photolithographic and Soft Lithographic Techniques
Jonas Christoffersson and Carl-Fredrik Mandenius
22. Using a Microfluidic Device for Culture and Drug Toxicity Testing of 3D Cells
Jonas Christoffersson and Carl-Fredrik Mandenius
23. hiPS Cell–Derived Neurons for High-Throughput Screening
Stefanie Traub and Ralf Heilker
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 08-2020
Ouvrage de 266 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 121,31 €
Ajouter au panierDate de parution : 05-2019
Ouvrage de 266 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 168,79 €
Ajouter au panier