Nucleic Acid Detection, Softcover reprint of the original 1st ed. 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 1039

In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

Part I: Advances in Sample Preparation

1. “Getting Blood from a Stone”: Ultra-Sensitive Forensic DNA Profiling of Microscopic Bio-particles Recovered from ‘Touch DNA’ Evidence

Erin K. Hanson and Jack Ballantyne

2. Detecting MicroRNA in Human Cancer Tissues with Fluorescence in situ Hybridization

Zonggao Shi, Jeff J. Johnson, and M. Sharon Stack

 Part II: New Real-Time and Instantaneous Assays

3. MN Azyme qPCR: A Superior Tool for Multiplex qPCR

Elisa Mokay and Alison V. Todd

4. A New, Multiplex, Quantitative Real-time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification

Fang Liang, Neetika Arora, Kang Liang Zhang, David Che Cheng Yeh, Richard Lai, Darnley Pearson, Graeme Barnett, David Whiley, Theo Sloots, Simon R. Corrie, and Ross T. Barnard

5. Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with Universal Molecular Beacon Reporter

Yulia V. Gerasimova, Jack Ballantyne, and Dmitry M. Kolpashchikov

6. SNP Analysis using a Molecular Beacon-based Operating Cooperatively (OC) Sensor

Evan M. Cornett and Dmitry M. Kolpashchikov

 Part III: Isothermal Amplification Methods for Nucleic Acid Detection

7. Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification

Wanyuan Ao and Robert Jenison        

8.      Rapid Detection of Brucella spp. using Loop-mediated Isothermal Amplification (LAMP)

Shouyi Chen, Xunde Li, Juntao Li, and Edward R. Atwill

9.       Loop-mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms

Yasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, and Akira Ito

10.   Detection of Mutation by Allele-specific Loop-mediated Isothermal Amplification (AS-LAMP)

Hiroka Aonuma, Athanase Badolo , Kiyoshi Okado, and Hirotaka Kanuka

Part IV: Signal Amplification Approac

11.      DNA Detection by Cascade Enzymatic Signal Amplification

Bingjie Zou, Yinjiao Ma and Guohua Zhou

Part V: Non-Fluorescent Detection Formats

12.  Visual DNA Detection and SNP Genotyping using Asymmetric PCR and Split DNA Enzymes

Jia Ling Neo and Mahesh Uttamchandani

13.      Detection of Single-stranded Nucleic Acids via Colorimetric Means, using G-quadruplex Probes

Shizuka Nakayama and Herman O. Sintim

14.       Lateral Flow Biosensors for the Detection of Nucleic Acid

Lingwen Zeng, Puchang Lie, Zhiyuan Fang, Zhuo Xiao

15.     Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a

Wireless Bipolar Electrode

Mei-Sheng Wu, Guang-sheng Qian, Jing-Juan Xu, and Hong-Yuan Chen

16.      High-throughput Label-free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging Sensor

Sunmin Ahn, David S. Freedman, Xirui Zhang, and M. Selim Ünlü¹

 Part VI: Advances in Fish

17.     Graphene -PAMAM Dendrimer -Gold Nano Particle Composite for Electrochemical DNA Hybridization Detection

Kumarasamy Jayakumar,  Rajendiran Rajesh ,Venkataraman Dharuman, and Rangarajan Venkatesan

18.  Application of PNA Openers for Fluorescence-based Detection of Bacterial DNA

Irina Smolina

19.  DNA probes for FISH analysis of C-Negative Regions in Human Chromosomes

Evgeniy S. Morozkin, Tatyana V. Karamysheva, Pavel P. Laktionov, Valentin V. Vlassov, and Nikolay B. Rubtsov

 Part VII: Detection of microRNA

20. Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary Electrophoresis

Nasrin Khan and Maxim V. Berezovski

 21. High-Throughput Functional MicroRNA Profiling using Recombinant AAV-Based MicroRNA Sensor Arrays

Wenhong Tian, Xiaoyan Dong, Xiaobing Wu, and Zhijian Wu

22.  The Use of Molecular Beacons to Detect and Quantify MicroRNA

Meredith B. Baker, Gang Bao, and Charles D. Searles

 Part VIII: RNA Imaging in Live Cells

23.         Sequence Specific Imaging of Influenza A mRNA in Living Infected Cells Using Fluorescent FIT-PNA

Susann Kummer, Andrea Knoll, Andreas Herrmann, and Oliver Seitz

24.      Application of Caged Fluorescent Nucleotides to Live-cell RNA Imaging

Akimitsu Okamoto

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras