Nanoimaging, 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 950

For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology.  Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales.  Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution.  The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography;  and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging.  Written in the successful format of the Methods in Molecular Biology? series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.

Introduction: Nanoimaging Techniques in Biology.- Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy.- 4Pi Microscopy.- Fluorescence in situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy.- Two-Color STED Imaging of Synapses in Living Brain Slices.- Super-Resolution Imaging by Localization Microscopy.- High-Content Super-Resolution Imaging of Live Cell by uPAINT.- Super-Resolution Fluorescence Imaging with Blink Microscopy.- Photoswitchable Fluorophores for Single-Molecule Localization Microscopy.- Single-Molecule Tracking of mRNA in Living Cells.- Semiautomatic, High-Throughput, High-Resolution Protocol for Three-Dimensional Reconstruction of Single Particles in Electron Microscopy.- Mass Mapping of Amyloid Fibrils in the Electron Microscope using STEM Imaging.- Elemental Mapping by Electron Energy Loss Spectroscopy in Biology.- Cellular Nanoimaging by Cryo Electron Tomography.- Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by SEM-Based Scanning Transmission Electron Microscopy.- 3D Imaging of Cells and Tissues by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM).- Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy.- Atomic Force Microscopy Imaging of Macromolecular Complexes.- Imaging of Transmembrane Proteins Directly Incorporated Within Supported Lipid Bilayers Using Atomic Force Microscopy.- Functional AFM Imaging of Cellular Membranes Using Functionalized Tips.- Near-Field Scanning Optical Microscopy for High Resolution Membrane Studies.- Correlative Fluorescence and EFTEM Imaging of the Organized Components of the Mammalian Nucleus.- High Data Output Method for 3-D Correlative Light-Electron Microscopy Using Ultrathin Cryosections.- Correlative Optical and Scanning Probe Microscopies for Mapping Interactions at Membranes.- Nanoimaging Cells Using Soft X-Ray Tomography.- Secondary Ion Mass Spectrometry Imaging of Biological Membranes at High Spatial Resolution.