MicroRNA and Cancer, 2011 Methods and Protocols Methods in Molecular Biology Series, Vol. 676

The tiny microRNAs (miRNAs) can have huge impacts on the regulation of a variety of genes and play crucial roles in the fundamental cellular processes. Recent miRNA studies change the landscape of cancer genetics by scrutinizing the alterations of genome-wide miRNA expressions in most common cancers and their regulatory functions during the development of cancer. The connections between miRNAs and cancer are widespread enough to warrant more comprehensive investigations in the systems biology perspective. In MicroRNA and Cancer: Methods and Protocols, internationally renowned experts provide the latest miRNA knowledge, the various techniques and methodologies currently available for cancer research application. Ranging from the fundamental concepts to practical applications, this book presents: ? Overview of microRNA biogenesis, computational prediction of new miRNAs in the cancer genome, and miRNA-based therapeutic approaches for cancer treatment ? Detailed experimental protocols in miRNA detection with novel and high-throughput technology, miRNA library cloning, miRNA epigenetic regulation, and miRNA pathway study ? Stepwise computational and bioinformatic procedures for miRNA complex networks in cancer genomes with a variety of softwares and programs ? Cross-cited notes on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols serves researchers with the basic principles of experimental and computational methods for microRNA study in cancer research and provides a firm grounding for those who wish to further develop their own applications and tailor them to their own specific research needs.

MicroRNA Biogenesis and Cancer.- Computational Identification of MiRNAs Involved in Cancer.- The Principles of MiRNA-Masking Antisense Oligonucleotides Technology.- The Concept of Multiple-Target Anti-miRNA Antisense Oligonucleotide Technology.- Modulation of MicroRNAs for Potential Cancer Therapeutics.- Detection of MicroRNAs in Cultured Cells and Paraffin-Embedded Tissue Specimens by In situ Hybridization.- MicroRNA Northern Blotting, Precursor Cloning, and Ago2-Improved RNA Interference.- MiRNA Profiling on High Throughput OpenArray™ System.- Silicon Nanowire Biosensor for Ultrasensitive and Label-Free Direct Detection of miRNAs.- High Throughput and Reliable Protocols for Animal microRNA Library Cloning.- MicroRNA Regulation of Growth Factor Receptor Signalling in Human Cancer Cells.- Epigenetic Regulation of MicroRNA Expression in Cancer.- In vitro Functional Study of miR-126 in Leukemia.- Prediction of the Biological Effect of Polymorphisms within MicroRNA Binding Sites.- The Guideline of the Design and Validation of MiRNA Mimics.- Analysis of Targets and Functions Co-Regulated by MicroRNAs.- Utilization of SSCprofiler to Predict a New MiRNA Gene.- MicroRNA-Profiling Using Fluorescence-Labeled Beads: Data Acquisition and Processing.

Highlights the fast-growing field of microRNAs with the pivotal, cutting-edge knowledge of its relation to cancer biology Provides contributions from experts, including their invaluable experience in the form of notes and tips from the lab Serves as a practical guide for scientists of all levels of study Includes supplementary material: sn.pub/extras