Sorghum, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1931

Langue : Anglais

Coordonnateurs : Zhao Zuo-Yu, Dahlberg Jeff

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This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study. Chapters are divided into five review chapters, five case study chapters, and nine protocol chapters providing comprehensive reviews, new study results or state-of-the-art protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Sorghum: Methods and Protocols aims to provide useful information and tools to an array of readers looking to research and utilize sorghum.

Hybrid Sorghum Product Development and Production.- Mapping QTLs and Identification of Genes Associated with Drought Resistance in Sorghum.- Field Studies to Develop Weed Management Programs for Grain Sorghum.- Discovery of Sorghum Haploid Induction System.- Development of a Pedigreed Sorghum Mutant Library.- Marker Assisted Selection in Sorghum using KASP Assay for Detection of Single Nucleotide Polymorphism/Insertion Deletion.- Assaying Sorghum Nutritional Quality.- Tannin Analysis in Sorghum Grains.- Sorghum Phytochemicals and their Potential Impact on Human Health.- Identification and Quantification of Carotenoids and Tocochromanols in Sorghum Grain by High Performance Liquid Chromatography.- Gene Editing in Sorghum through Agrobacterium.- Genome Editing by CRISPR/Cas9 in Sorghum through Biolistic Bombardment.- Novel Ternary Vectors for Efficient Sorghum Transformation.- Nutritionally Enhanced Sorghum for the Arid and Semi-Arid Tropical Areas of Africa.- Evaluation of Agronomic Performance of β-Carotene Elevated Sorghum in Confined Field Conditions.- Economics of Increasing Sorghum Productivity in Sub-Saharan Africa: The Mali Case.- Background on Grain Sorghum Usage.- Assaying Sorghum for Fuel Production.- The Role of Sorghum in Renewables and Biofuels.

Includes cutting-edge methods and protocols provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts