Recombinant Proteins from Plants (2^nd Ed., Softcover reprint of the original 2nd ed. 2016) Methods and Protocols Methods in Molecular Biology Series, Vol. 1385

This volume provides up-to-date scientific achievements from the world?s top researchers. RecombinantProteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, RecombinantProteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.

Part I: Vectors and Transformation

1. Chloroplast Based Expression of Recombinant Proteins by Gateway® Cloning Technology

Johanna Gottschamel and Andreas Lössl                                                                              

2.  Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors

Ruifeng He

3. Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

Pooja Saxena, Eva C. Thuenemann, Frank Sainsbury, and George P. Lomonossoff

4. Transient Protein Expression by Agroinfiltration In Lettuce

Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa Mcnulty, Kahlin Leuzinger, Huafang Lai

5. Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas Reinhardtii          

Daniel Guzmán-Zapata, Karla Soledad Macedo-Osorio, Alma Lorena

Part II: Product Improvements

6.  Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes

Nicoletta Ferradini, Angelica Giancaspro, Alessandro Nicolia, Agata Gadaleta, Fabio Veronesi, Daniele Rosellini

7. Transient Expression of Mammalian Genes In Nicotiana Benthamiana To Modulate N-Glycosylation  

Alexandra Castilho

8. Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants

Stéphanie Robert, Philippe V. Jutras, Moustafa Khalf, Marc-André D’Aoust, Marie-Claire Goulet, Frank Sainsbury and Dominique Michaud 

Part III: Extraction and Scale-Up

9. Production of Recombinant Cholera Toxin B Subunit in Nicotiana Benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector

Lauren Moore, Krystal Hamorsky, and Nobuyuki

10. Total Soluble Protein Extraction for Improved Proteomic Analysis Of Transgenic Plant Roots 

Manish L. Raorane, Joan O. Narciso, and Ajay Kohli

11. Temporary Immersion Bioreactors for the Contained Production Of Recombinant Proteins In Transplastomic Plants

Sherwin Barretto, Franck Michoux, and Peter J. Nixon

12. Plant Cell-Based Recombinant Antibody Manufacturing with A 200 L Orbitally Shaken Disposable Bioreactor                                                                                                                                                

Nicole Raven, Stefan Schillberg, and Stefan Rasche

13. Liquid-Liquid Phase Separation of Oil Bodies From Seeds                                                                      

Cory L. Nykiforuk

14. Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture

Lauri J. Reuter, Andrew J. Conley, and Jussi J. Joensuu

Part IV: Analysis

15. Molecular Analyses of Transgenic Plants

Kurniawan Rudi Trijatmiko, Felichi Mae Arines, Norman Oliva, Inez Slamet-Loedin, and Ajay Kohli

16. Analysis of Recombinant Proteins In Transgenic Rice Seeds: Identity, Localization, Tolerance To Digestion, And Plant Stress Response

Yuhya Wakasa and Fumio Takaiwa

17. Real-Time PCR-Based Quantitation Method for The Genetically Modified Soybean Line GTS 40-3-2

Kazumi Kitta, Reona Takabatake, and Junichi Mano

18. A Standardized Lepidopteran Bioassay to Investigate the Bioactivity Of Insecticidal Proteins Produced In Transgenic Crops

Gerson Graser and Frederick S. Walters

Part V: Antibodies for Pathogen Resistance

19. Generating Transgenic Plants

Dieter Peschen, Stefan Schillberg, and Rainer Fischer

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras