G Protein-Coupled Receptor Signaling in Plants, 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 1043

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins.  Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs.- Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins.- Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana.- Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants.- Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis.- Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis.- Fusarium oxysporum Infection Assays in Arabidopsis.- Analysis of Unfolded Protein Response in Arabidopsis.- Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis.- G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings.- Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana.- In Vivo Localization in Arabidopsis Protoplasts and Root Tissue.- Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography–Coupled Mass Spectrometry.- In Vitro Myristoylation Assay of Arabidopsis Proteins.- Assaying Protein S-Acylation in PlantIn Vitro Prenylation Assay of Arabidopsis Proteins.