Zika Virus, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2142

This volume discusses the latest protocols used by scientists to understand ZIKV and how to mitigate its presence. These studies will benefit in the detection, diagnosis, management, treatment, and prevention of this infection. The chapters in this book cover techniques in virus isolation, purification, and ways to titrate the virus; recent advancements made in the development of modified molecular techniques; and a discussion on Zika virus itself to help readers better understand this pathogen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Zika Virus: Methods and Protocols is a valuable resource for both novice and expert scientists interested in learning more about this important topic. 

Preface…
Table of Contents…
Contributing Authors…

1. Zika Virus: A Brief History and Review of its Pathogenesis Rediscovered
Joel N. Maslow and Christine C. Roberts

2. Zika Virus Isolation, Purification, and Titration
Mariana Baz

3. Evaluating Zika Virus Pathogenesis in Immunocompromised Mice
Bryan D. Griffin, David Safronetz, and Darwyn Kobasa

4. Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice
William Enlow, Jocelyne Piret, and Guy Boivin

5. A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies
Kristina Dimitrova, Emelissa J. Mendoza, Nicole Barairo, and Heidi Wood

6. Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test
Jessica L. Smith and Alec J. Hirsch

7. Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation
Raquel das Neves Almeida, Heloisa Antoniella Braz-de-Melo, Rafael Corrêa, and Kelly Grace Magalhães

8. Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells 
Young Chan Kim, Nallely Garcia-Larragoiti, Cesar Lopez-Camacho, Martha Eva Viveros-Sandoval, and Arturo Reyes-Sandoval 

9. Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA
Young Chan Kim, Maud Dumoux, Raymond J. Owens, and Arturo Reyes-Sandoval 

10. Zika Virus Peptide ELISA (ZIKV-NS2B-concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection
Nischay Mishra, Riddhi Thakkar, James Ng, and W. Ian Lipkin

11. Zika Virus Amplification using Strand Displacement Isothermal Method and Sequencing using Nanopore Technology
Sören Hansen, Oumar Faye, Sabri S. Sanabani, Martin Faye, Susanne Böhlken-Fascher, Ousmane Faye, Amadou A. Sall, Michaël Bekaert, Manfred Weidmann, Claus-Peter Czerny, and Ahmed Abd El Wahed

12. Detection of Zika Virus using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP)
Laura E. Lamb, Sarah N. Bartolone, and Michael B. Chancellor

13. Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus
Benjamin Lopez-Jimena, Mohammed Bakheit, Michaël Bekaert, Graham Harold, Sieghard Frischmann, Cheikh Fall, Cheikh Tidiane Diagne, Oumar Faye, Ousmane Faye, Amadou Alpha Sall, and Manfred Weidmann

14. Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA
Cheikh Tidiane Diagne, Martin Faye, Benjamin Lopez-Jimena, Ahmed Abd El Wahed, Cheikh Loucoubar, Cheikh Fall, Giulia Mencatelli, Oumar Faye, Ousmane Faye, Manfred Weidmann, and Amadou Alpha Sall

15. A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response
Ivan Trus, Stewart Walker, Maria Fuchs, Daniel Udenze, Volker Gerdts, and Uladzimir Karniychuk 

16. Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes
Nicole Haese, Alec J. Hirsch, and Daniel N. Streblow

17. Discovery of Zika Virus Dependency and Restriction Factors using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT)
William M. McDougall, Manish Kandpal, Jill M. Perreira, and Abraham L. Brass

18. Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy 
Nabila Jabrane-Ferrat and Francisco Veas

19. Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain
Daniel Limonta, William Branton, Cheung Pang Wong, Leina Saito, Christopher Power, and Tom C. Hobman

20. Isolation of Monoclonal Antibodies from Zika Virus-Infected Patient Samples
Qihui Wang and Jinghua Yan

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts