Two-Hybrid Systems, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1794

1. Protein Interactomics by Two-Hybrid Methods

            Soon Gang Choi, Aaron Richardson, Luke Lambourne, David E. Hill, and Marc Vidal

Part I: Yeast Hybrid Systems to Study Protein, Nucleic Acid, and Ligand Interactions

2. Making the Right Choice: Critical Parameters of the Y2H Systems

            Jitender Mehla, J. Harry Caufield, and Peter Uetz

3. Identification of Protein-Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening

            Thomas Lathouwers, Jeroen Wagemans, and Rob Lavigne

4. Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System

            Lisa Yasmin Asseck and Christopher Grefen

5. The Ras Recruitment System (RRS) for the Identification and Characterization of Protein-Protein Interactions

            Ami Aronheim

6. A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning

            Macy G. Olson, Megan Goldammer, Emilie Gauliard, Daniel Ladant, and Scot P. Ouellette

7. Analyses of Protein Interaction Networks Using Computational Tools

            Shaowei Dong and Nicholas J. Provart

8. Options and Considerations When Using a Yeast One-Hybrid System

            Jared A. Sewell and Juan I. Fuxman Bass

9. Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins

            Rocío Sánchez-Montesino and Luis Oñate-Sánchez

10. Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens

            S. Earl Kang, Ghislain Breton, and Jose L. Pruneda-Paz

11. Transcription Factor-Centered Yeast One-Hybrid Assay

            Xiaoyu Ji, Liuqiang Wang, Dandan Zang, and Yucheng Wang

12. The Yeast Three-Hybrid System for Protein Interactions

            Franziska Glass and Mizuki Takenaka

13. The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants

            Sung Ki Cho and David J. Hannapel

14. Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules

            You Wang, David S. Letham, Peter C.L. John, and Ren Zhang

15. Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System

            Alexander Heyl

Part II: Experimental Approaches to Study Protein-Protein and Protein-DNA Interactions in Plants and Human Cells

16. Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells

            Shino Goto-Yamada, Kazumi Hikino, Mikio Nishimura, Tsuyoshi Nakagawa, and Shoji Mano

17. Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Human Cells Based on Gateway Cloning Technology

            Adriana Lepur and Oliver Vugrek

18. KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein-Protein Interactions

            Delphine Masschaele, Sarah Gerlo, Irma Lemmens, Sam Lievens, and Jan Tavernier

19. Coimmunoprecipitation of Interacting Proteins in Plants

            Alfonso Muñoz and M. Mar Castellano

20. Analysis of Protein-Protein Interaction by Co-IP in Human Cells

            Zhenyuan Tang and Yoshinori Takahashi

21. Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures

            Marta García-León, Elisa Iniesto, and Vicente Rubio

22. Transient Transactivation Studies in Nicotiana benthamiana Leaves

23. The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions

            José A. Jarillo, Dorota N. Komar, and Manuel Piñeiro

24. Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells

            Bjørk Ditlev Larsen, Martin Rønn Madsen, Ronni Nielsen, and Susanne Mandrup

Includes cutting-edge methods and protocols for the study of two-hybrid systems

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts