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Tissue Morphogenesis, Softcover reprint of the original 1st ed. 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1189

Langue : Anglais

Coordonnateur : Nelson Celeste M.

Couverture de l’ouvrage Tissue Morphogenesis

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.


 Chapter 1.       Probing Regional Mechanical Properties of Embryonic Tissue Using                                                Microindentation and Optical Coherence Tomography

Benjamin A. Filas, Gang Xu, and Larry A. Taber


Chapter 2.       Hemodynamic Flow Visualization of Early Embryonic Great Vessels using mPIV

Selda Goktas, Chia-Yuan Chen, William J. Kowalski, and Kerem Pekkan


Chapter 3.       Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis

Jacky G. Goetz, Fabien Monduc, Yannick Schwab, and Julien Vermot


Chapter 4.       Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis

 Chelsea L. Gregg, Andrew K. Recknagel, and Jonathan T. Butcher


Chapter 5.       Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila Melanogaster Embryos

Sophia N. Carrell and Gregory T. Reeves


Chapter 6.       Light-Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly

Khaled Khairy, William Lemon, Fernando Amat, and Philipp J. Keller


Chapter 7.       Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis

 Chun Yin Bosco Leung and Rodrigo Fernandez-Gonzalez


Chapter 8.       A Multiplex Fluorescent in situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis

 Lily S. Cheung and Stanislav Shvartsman


Chapter 9.       Active Cell and ECM Movements During Development

Anastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, and Andras Czirok



 Chapter 10.     3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion

Kim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner, Jennifer N. Beck, Kevin J. Cheung, and Andrew J. Ewald

 Chapter 11.     Culture of Mouse Embryonic Foregut Explants

 Felicia Chen and Wellington V. Cardoso

 Chapter 12.     Investigating Human Vascular Tube Morphogenesis and maturation using  endothelial cell-pericyte co-cultures and a doxycycline-inducible genetic system in 3D extracellular matrices

 Stephanie L.K. Bowers, Chun-Xia Meng, Matthew T. Davis, and George E. Davis

 Chapter 13.     Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues

 Alexandra S. Piotrowski, Victor D. Varner, Nikolce Gjorevski, and Celeste M. Nelson



Chapter 14.     Probing Cell Mechanics with Sub-Cellular Laser Dissection of Actomyosin  Networks in the Early Developing Drosophila Embryo

 M. Rauzi and P.-F. Lenne


Chapter 15.     UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish    Embryo in vivo and ex vivo

Michael Smutny, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp Heisenberg


Chapter 16.     Measurement of Intercellular Cohesion by Tissue Surface Tension

 Ramsey A. Foty


Chapter 17.     Quail-Chick Chimeras and Eye Development

 Sinu Jasrapuria Agrawal and Peter Y. Lwigale



 Chapter 18.     Studying Epithelial Morphogenesis in Dictyostelium

 Daniel J. Dickinson, W. James Nelson, and William I. Weis


Chapter 19.     Primary Cell Cultures of Regenerating Holothurian Tissues

 Samir A. Bello, Ricardo J. Abreu-Irizarry, and Jose E. Garcia-Arraras



 Chapter 20.     Large-Scale Parameter Studies of Cell-Based Models of Tissue Morphogenesis   Using CompuCell3D or VirtualLeaf

Margriet M. Palm and Roeland M. H. Merks


Chapter 21.     Simulating Tissue Morphogenesis and Signaling

Dagmar Iber, Simon Tanaka, Patrick Fried, Philipp Germann, and Denis  Menshykau


Chapter 22.     Elasticity-Based Targeted Growth Models of Morphogenesis

 Patrick W. Alford

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Date de parution :

Ouvrage de 351 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 116,04 €

Ajouter au panier

Date de parution :

Ouvrage de 351 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 168,79 €

Ajouter au panier
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