The Golgi Complex, 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1496

1. 4D Confocal Imaging of Yeast Organelles

  Kasey J. Day, Effrosyni Papanikou, and Benjamin S. Glick

2. Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons

  Ginny G. Farías, Dylan J. Britt, and Juan S. Bonifacino

3. Imaging Golgi Outposts in Fixed and Living Neurons

   Mariano Bisbal, Gonzalo Quassollo, and Alfredo Caceres

4. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor

   Jon E. Paczkowski and J. Christopher Fromme

5. STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells

   Byung-Ho Kang

6. Reconstitution of COPI Vesicle and Tubule Formation

   Seung-Yeol Park, Jia-Shu Yang, and Victor W. Hsu

   Edward B. Cluett, Paul de Figueiredo, Marie E. Bechler, Kevin D. Thorsen, and William J. Brown

8. Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures

   Sara Fasmer Hansen, Berit Ebert, Carsten Rautengarten, and Joshua L. Heazlewood

9. High Content Analysis of the Golgi Complex by Correlative Screening Microscopy

   Manuel Gunkel, Holger Erfle, and Vytaute Starkuviene

10. Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo

    Lina Song, Collin Bachert, and Adam D. Linstedt

11. In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures

   Dorota Maszczak-Seneczko, Paulina Sosicka, Teresa Olczak, and Mariusz Olczak

12. Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function

   Jessica Bailey Blackburn and Vladimir V. Lupashin

13. Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway

   Riccardo Rizzo and Alberto Luini

14. Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle

  Inmaculada Ayala and Antonino Colanzi

15. The Role of Lysophospholipid Acyltransferases in the Golgi Complex

   John A. Schmidt

16. Methods to Purify and Assay Secretory Pathway Kinases

   Vincent S. Tagliabracci, Jianzhong Wen, and Junyu Xiao

Includes cutting-edge methods and protocols for Golgi complex research

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras