Teratogenicity Testing (2^nd Ed., 2nd ed. 2024) Methods and Protocols Methods in Molecular Biology Series, Vol. 2753

Part I: Introduction to Teratology Testing

1. Updating an Overview of Teratology

            Ana Margarida Calado, Fernanda Seixas, and Maria dos Anjos Pires

2. Guidelines on Development Toxicity Tests: Brief Insights

3. Development Features on the Selection of Animal Models for Teratogenic Testing

            Sofia Alves-Pimenta, Bruno Colaço, Paula A. Oliveira, and Carlos Venâncio

4. Virus as Teratogenic Agents

            Ana Margarida Calado, Fernanda Seixas, and Maria dos Anjos Pires

5. The Future of the Teratogenicity Testing

            Muhsin Aydin, Banu Guven Ezer, and Eyyup Rencuzogullari

6. The First Steps on AOPs' Concepts, Development, and Applications in Teratology

            Leonardo Vieira, Terezinha Souza, and Davi Farias

7. Machine Learning to Predict Teratogenicity: Theory and Practice

            Latifa Douali

Part II: Experimental-Based Teratology Testing

8. Human Pluripotent Stem Cell-Based Assays to Predict Developmental Toxicity

            Madhura Banerjee, Aritrika Das, Prarthana Chatterjee, and Satarupa Banerjee

9. Developmental Toxicity Using the Rat Whole Embryo Culture

            Zeliha Fazliogullari

10. A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs

            Mahshid Hodjat and Mohammad Abdollahi

11. Teratogenic Effects of Drugs on Primary Lymphocytes Assessed by Flow Cytometry

            Ahmad Salimi and Zhaleh Jamali

12. Assessment of the Teratogenic Effect of Drugs on the Chicken Embryo

            Lucas Rosa Fraga, Maikel Rosa de Oliveira, Karina Maria Wermann, and Neil Vargesson

13. Daphnia magna as a Model Organism to Predict the Teratogenic Effect of Different Compounds

            Monalisa Mishra

14. Caenorhabditis elegans as an In Vivo Model Organism to Elucidate Teratogenic Effects

            Zhenglu Wang

15. Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans after Developmental Lead Exposure

            Lucia E. Fernandez-Hubeid, Paula A. Albrecht, Michael Aschner, and Miriam B. Virgolini

16. Exploration of Teratogenic and Genotoxic Effects on Model Organism Drosophila melanogaster

            Seekha Naik and Monalisa Mishra

17. Ecotoxicological Assessment of Seaweed-Based Crop Biostimulant on Earthworm Eudrilus eugeniae Kinb

            Kanagaraj Muthu-Pandian Chanthini and Sengottayan Senthil-Nathan

18. Evaluation of the Ecotoxicology of Seaweed-Based Biopesticide Used in Combat of the Polyphagous Pest Using Eudrilus eugeniae Kinb

            Kanagaraj Muthu-Pandian Chanthini, Haridoss Sivanesh, Ramakrishnan Ramasubramanian, Vethamonickam Stanley-Raja, Sengodan Karthi, Narayanan Shyam-Sundar, and Sengottayan Senthil-Nathan

19. Biochemical Studies to Understand Teratogenicity and Lethality Outcomes in Modified-FETAX

            Abbas Güngördü and Duygu Ozhan Turhan

20. Bioinformatics Methods for Transcriptome Analysis on Teratogenesis Testing

            Thayne Woycinck Kowalski, Giovanna Câmara Giudicelli, Julia do Amaral Gomes, Mariana Recamonde-Mendoza, and Fernanda Sales Luiz Vianna

21. Angiogenesis Assay for Live and Fixed Zebrafish Larvae

            Vinoth S, Satheeswaran Balasubramanian, Ekambaram Perumal, and Kirankumar Santhakumar

22. High-Resolution Respirometry for the Assessment of Teratogenic Chemicals

            Christopher L. Souders II, Isaac Konig, and Christopher J. Martyniuk

23. A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae

            Dércia Santos, Sandra M. Monteiro, and Ana Luzio

24. Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos

            Mine Köktürk and Fikret Altındağ

25. Assessment of Developmental Neurotoxicity Using Semi-Automatic Behavior Analysis System for Zebrafish

            Derya Cansız, İsmail Ünal, Merih Beler, A. Ata Alturfan, and Ebru Emekli-Alturfan

26. Behavioral Profiling of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle Response

            Ana M. Valentim

27. Alcian Blue Staining for Chondrocranium Development in Zebrafish

            Merih Beler, İsmail Ünal, Derya Cansız, and Ebru Emekli-Alturfan

28. Protocol of Geometric Morphometrics for Teratogenicity Testing

            Mariana Caipira Lei, Luís Félix, and Carlos Venâncio

29. Biochemical Markers for Liver Injury in Zebrafish Larvae

            Rafael Xavier Martins, Juliana Alves Costa Ribeiro Souza, Maria Eduarda Maia, Matheus Carvalho, Terezinha Souza, and Davi Farias

30. Cortisol Quantification for Assessing Stress-Induced Changes in Zebrafish Larvae

            Raquel S.F. Vieira, Carlos Venâncio, and Luís Félix

31. Metabolomic Fingerprint Assay in Zebrafish Embryos

            Theodoros G. Chatzimitakos, Ioannis Leonardos, and Constantine D. Stalikas

32. Alkaline Comet Assay to Assess Genotoxicity in Zebrafish Larvae

            Ondina Ribeiro, Isabel Gaivão, and João Soares Carrola

33. A Chromogenic Quantification of Protein Expression in Zebrafish Larvae

            Luís Félix

34. Confocal Microscopy Technique in Teratogenicity Testing Using Zebrafish (Danio rerio) Embryos as Model

            Thangal Yumnamcha, Reema Khangembam, Sangeeta Yanglem, Kshetrimayum Ophelia, Sonali Thokchom, and Damayanti Maibam

35. Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos

            İsmail Ünal, Derya Cansız, Merih Beler, A. Ata Alturfan, and Ebru Emekli-Alturfan

36. Quantitative Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos

            Merih Beler, Derya Cansız, İsmail Ünal, and Ebru Emekli-Alturfan

37. Microarray Analysis to Determine Gene Expression Changes in Zebrafish Embryos

            İsmail Ünal, Merih Beler, Derya Cansız, and Ebru Emekli-Alturfan

38. Cell-Free DNA and Next-Generation Sequencing for Prenatal Diagnosis

            Klaus Rieneck

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts