Techniques in Quantification and Localization of Gene Expression, Softcover reprint of the original 1st ed. 2000

Can the son or daughter of a baseball pitcher or cricket bowler throw a ball 100 miles an hour? Is the son or daughter of an opera singer also an opera singer? Is a house with functional light switches lit? The line of thinking in these rhetorical questions also applies to human genetics. What do baseball pitchers, opera sing­ ers, light switches, and the Human Genome Project have in common? These questions address the issue of potential versus realization of function. Although sons and daughters of baseball pitchers and opera singers may have inherited the mechanical attributes to be baseball pitchers and opera singers, they may not, at any point in time, be baseball pitchers or opera singers. A house with functional light switches is not lit unless the light switches are on. Similarly, all of the genes discovered and sequenced as a result of the Human Genome Project are not expressed at the same time. Genome project information will allow us to deter­ mine the repertoire of genes in an individual, which is analogous to determining where the light switches in a house are located and whether they are functional (a mutation or deletion in the Genome Project Model). The pattern of "on" light switches in a house gives us functional information as to what the family inside is doing (e. g. , eating, reading, sleeping). Similarly, the pattern of gene expression (RNA) gives us information on what our bodies are doing (e. g.

Basic Methods.- 1. Basic Flow Cytometry Instrumentation.- 2. Fixation for In Situ Molecular Analysis.- 3. A Method for Reduction of Green Wavelength Autofluorescence Emission in Cellular Preparations.- 4. Gene Quantification: Choosing the Target.- 5. Detection and Quantification of Cytokine-Producing Cells by Immunostaining.- 6. In Situ Hybridization Using the bDNA Technology.- Specific Applications.- 7. Kaposi’s Sarcoma—Associated Human Herpes Virus Eight: Localization of Viral Gene Expression and Viral Latency.- 8. Pathogenetic Mechanisms of Animal Orbiviruses That Cause Disease at Low Copy Number.- 9. Localization of Hepatitis C Virus Using Reverse Transcriptase Fluorescence In Situ 5’ Nuclease Assay.- 10. Simultaneous Quantification and Localization of HIV gag-pol Expression Using Ultrasensitive Fluorescence In Situ Hybridization.- 11. Quantification and Localization of Gene Expression: Future Directions.