Synthetic Biology, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1772
Part I: Gene Circuits and Biochemical Pathways
1. Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria
Alberto Sánchez-Pascuala, Pablo I. Nikel, and Víctor de Lorenzo
2. Negative Regulation Gene Circuits for Efflux Pump Control
Daniel A. Charlebois, Junchen Diao, Dmitry Nevozhay, and Gábor Balázsi
3. Methods for Generating Microbial Co-Cultures that Grow in the Absence of Fixed Carbon or Nitrogen
Matthew J. Smith and Matthew B. Francis
4. Synthetic Biology with an All E. coli TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits
Ryan Marshall and Vincent Noireaux
5. Transformation of an Exotic Yeast Species into a Platform Organism: A Case Study for Engineering Glycolipid Production in the Yeast Starmerella bombicola
Sofie Lodens, Marilyn De Graeve, Sophie L.K.W. Roelants, Sofie L. De Maeseneire, and Wim Soetaert
6. Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux
Takuya Matsumoto, Tsutomu Tanaka, and Akihiko Kondo
Part II: Genome Editing and Modification
7. Construction of CRISPR Libraries for Functional Screening
Carsten-P. Carstens, Katherine A. Felts, and Sarah E. Johns
8. Targeted Genome Editing Using DNA-Free RNA-Guided Cas9 Ribonucleoprotein for CHO Cell Engineering
Jongoh Shin, Namil Lee, Suhyung Cho, and Byung-Kwan Cho
9. Dynamic Management of Codon Compression for Saturation Mutagenesis
Gur Pines and Ryan T. Gill
10. Designed Transcriptional Regulation in Mammalian Cells Based on TALE- and CRISPR/dCas9
Tina Lebar and Roman Jerala
11. Combinatorial Evolution of DNA with RECODE
Zhen Kang, Wenwen Ding, Peng Jin, Guocheng Du, and Jian Chen
12. Genome Editing in Penicillium chrysogenum Using Cas9 Ribonucleoprotein Particles
Carsten Pohl, László Mózsik, Arnold J.M. Driessen, Roel A.L. Bovenberg, and Yvonne Nygård
13. Targeted Gene Knockout Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1
Michael Gapinske, Nathan Tague, Jackson Winter, Gregory H. Underhill, Pablo Perez-Pinera
14. Programming Biomaterial Interactions Using Engineered Living Cells
Keith C. Heyde and Warren C. Ruder
15. Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems
Kaiwen Ivy Liu, Muhammad Nadzim Bin Ramli, Norfala-Aliah Binte Sutrisnoh, and Meng How Tan
16. Production of a Human Cell Line with a Plant Chromosome
Naoki Wada, Yasuhiro Kazuki, Kanako Kazuki, Toshiaki Inoue, Kiichi Fukui, and Mitsuo Oshimura
17. Bacterial Genome Editing with CRISPR-Cas9: Taking Clostridium beijerinckii as an Example
Zhong-Tian Zhang, Pablo Jiménez-Bonilla, Seung-Oh Seo, Ting Lu, Yong-Su Jin, Hans P. Blaschek, and Yi Wang
18. CRISPR-Cas9-Mediated Genome Editing and Transcriptional Control in Yarrowia lipolytica
Cory Schwartz and Ian Wheeldon
19. Construction and Integration of a Synthetic MicroRNA Cluster for Multiplex RNA Interference in Mammalian Cells
Tingting Wang and Zhen Xie
Part III: Genome Language and Computing
20. Bio-Algorithmic Workflows for Standardized Synthetic Biology Constructs
Angel Goñi-Moreno and Victor de Lorenzo
21. Engineering Genomes with Genotype Specification Language
Erin H. Wilson, Chris Macklin, and Darren Platt
22. Automated Visualization of Genetic Designs Using DNAplotlib
Vittorio Bartoli, Daniel O.R. Dixon, and Thomas E. Gorochowski
23. DNA-Based Analog Computing
Daniel Fu, Shalin Shah, Tianqi Song, and John Reif
Part IV: Molecular Assembly
24. DNA Assembly with the DATEL Method
Zhen Kang, Wenwen Ding, Peng Jin, Guocheng Du, and Jian Chen
25. EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology
Hung-En Lai, Simon Moore, Karen Polizzi, and Paul Freemont
26. Designing and Assembling Plasmids for Construction of Escherichia coli Biosensor for Vibrio cholerae Detection
Maciej B. Holowko and Chueh Loo Poh
27. A Rapid Combinatorial Approach to Assembling Synthetic Prokaryotic and Eukaryotic Protein Expression Vectors
Rebecca Mullinax, Sarah Johns, Denise Rhodes, Vivian Zhang, Nancy McKinney, Katie Felts, Carsten Carstens, and Peter Sheffield
28. Standardized Cloning and Curing of Plasmids
Ida Lauritsen, Se Hyeuk Kim, Andreas Porse, and Morten H.H. Nørholm
29. STAR: A Simple TAL Effector Assembly Reaction Using Isothermal Assembly
Sabine Gogolok, Ute Köber, and Steven M. Pollard
Includes cutting-edge methods and protocols for the study of synthetic biology
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 510 p.
17.8x25.4 cm
Date de parution : 05-2018
Ouvrage de 510 p.
17.8x25.4 cm