Structural Proteomics (3^rd Ed., 3rd ed. 2021) High-Throughput Methods Methods in Molecular Biology Series, Vol. 2305

Part I: Structural Bioinformatics

1. The Protein Data Bank Archive

            Sameer Velankar, Stephen K. Burley, Genji Kurisu, Jeffery C. Hoch, and John L. Markley

2. Computational Methods for the Elucidation of Protein Structure and Interactions

            Nicholas S. Edmunds and Liam J. McGuffin

3. Methods for Molecular Modeling of Protein Complexes

            Tejashree Rajaram Kanitkar,, Neeladri Sen, Sanjana Nair, Neelesh Soni, Kaustubh Amritkar, Yogendra Ramtirtha, and M.S. Madhusudhan

Part II: Protein Production

4. High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus

            Ester Behiels and Jonathan Elegheert

5. Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies

            Ganna O. Krasnoselska, Maud Dumoux, Nadisha Gamage, Harish Cheruvara, James Birch, Andrew Quigley, and Raymond J. Owens

6. Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells

            Maren Schubert, Manfred Nimtz, Federico Bertoglio, Stefan Schmelz, Peer Lukat, and Joop van den Heuvel

7. SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering

            Hannah Crocker, Barbara Gorda, Martin Pelosse, Deepak Balaji Thimiri Govinda Raj, and Imre Berger

8. Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification

            Sylvain Geny, Simon Pichard, Arnaud Poterszman, and Jean-Paul Concordet

            Marlitt Stech, Nathanaël Rakotoarinoro, Tamara Teichmann, Anne Zemella, Lena Thoring, and Stefan Kubick

Part III: Structure Determination

10. Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins

11. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources

            Pierre Aller and Allen M. Orville

12. From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example

            Christoph A. Diebolder, Rebecca S. Dillard, and Ludovic Renault

13. Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods

            Carlos Oscar S. Sorzano, Amaya Jiménez-Moreno, David Maluenda, Erney Ramírez-Aportela, Marta Martínez, Ana Cuervo, Robert Melero, Jose Javier Conesa, Ruben Sánchez-García, David Strelak, Jiri Filipovic, Estrella Fernández-Giménez, Federico de Isidro-Gómez, David Herreros, Pablo Conesa, Laura del Caño, Yunior Fonseca, Jorge Jiménez de la Morena, Jose Ramon Macías, Patricia Losana, Roberto Marabini, and Jose-Maria Carazo

14. Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection

            Ottilie von Loeffelholz and Bruno P. Klaholz

15. Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology

            Jana Moravcová, Radka Dopitová, Matyáš Pinkas, and Jiří Nováček

16. Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED

            Emma Danelius and Tamir Gonen

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts