Structural Proteomics (2^nd Ed., Softcover reprint of the original 2nd ed. 2015) High-Throughput Methods Methods in Molecular Biology Series, Vol. 1261

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes. Structural Proteomics: High-Throughput Methods, Second Edition begins by exploring the resources available for curation, annotation, and structure prediction in silico, and continues with methods for sample preparation of both proteins and crystals, as well as structural characterization techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Structural Proteomics: High-Throughput Methods, Second Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.

Part I: Bioinformatics and Data Management

1. Protein Structure Annotation Resources

            Margaret J. Gabanyi and Helen M. Berman

2. PiMS: A Data Management System for Structural Proteomics

            Chris Morris

3. Prediction and Analysis of Intrinsically Disordered Proteins

Marco Punta, István Simon, and Zsuzsanna Dosztányi

4. I-TASSER Suite: A Standalone Package for Protein Structure Prediction and Structure-Based Protein Function Annotation

            Renxiang Yan, Jianyi Yang, Ambrish Roy, Dong Xu, Jonathan Poisson, and Yang Zhang

Part II: Soluble Proteins

5. Characterization and Production of Protein Complexes by Co-Expression in Escherichia coli

            Matthias Haffke, Martin Marek, Martin Pelosse, Marie-Laure Diebold, Uwe Schlattner, Imre Berger, and Christophe Romier

6. The Production of Multi-Protein Complexes in Insect Cells Using the Baculovirus Expression System

            Wassim Abdulrahman, Laura Radu, Frederic Garzoni, Olga Kolesnikova, Kapil Gupta, Judit Osz-Papai, Imre Berger, and Arnaud Poterszman

7. Production of Cell Surface and Secreted Glycoproteins in Mammalian Cells

            Elena Seiradake, Yuguang Zhao, Weixian Lu, A. Radu Aricescu, and E. Yvonne Jones

8. Cell-Free Protein Synthesis Systems Derived from Cultured Mammalian Cells

            Andreas K. Brödel, Doreen A. Wüstenhagen, and Stefan Kubick

9. Crystallization: Digging into the Past to Learn Lessons for the Future

            Vincent J. Fazio, Thomas S. Peat, and Janet Newman

Part III: Membrane Proteins

10. Screening of Stable G Protein-Coupled Receptor Variants in Saccharomyces cerevisiae

            Mitsunori Shiroishi and Takuya Kobayashi

11. Cell-Free Expression of G Protein-Coupled Receptors

            Erika Orbán, Davide Proverbio, Stefan Haberstock, Volker Dötsch, and Frank Bernhard

12. GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System

            Nien-Jen Hu, Heather Rada, Nahid Rahman, Joanne E. Nettleship, Louise Bird, So Iwata, David Drew, Alex Cameron, and Raymond J. Owens

13. Methods for the Successful Crystallization of Membrane Proteins

            Isabel Moraes and Margarida Archer

Part IV: Structural Characterization of Proteins

14. Application of In Situ Diffraction in High-Throughput Structure Determination Platforms

            Pierre Aller, Juan Sanchez-Weatherby, James Foadi, Graeme Winter, Carina M.C. Lobley,  Danny Axford, Alun W. Ashton, Domenico Bellini, Jose Brandao-Neto, Simone Culurgioni, Alice Douangamath, Ramona Duman, Gwyndaf Evans, Stuart Fisher, Ralf Flaig, David R. Hall, Petra Lukacik, Marco Mazzorana, Katherine E. McAuley, Vitaliy Mykhaylyk, Robin L. Owen, Neil Paterson, Pierpaolo Romano,  James Sandy, Thomas Sorensen, Frank von Delft, Anna Warren, Mark Williams, David I. Stuart, and Martin A. Walsh

15. CD Spectroscopy: An Essential Tool for Quality Control of Protein Folding

            Giuliano Siligardi and Rohanah Hussain

16. High Throughput Studies of Protein Shapes and Interactions by Synchrotron Small-Angle X-Ray Scattering

            Cy M. Jeffries and Dmitri I. Svergun

17. Automated Structure Determination from NMR Spectra

            Elena Schmidt and Peter Güntert

18. Solid State Nuclear Magnetic Resonance Spectroscopy for Membrane Protein Structure Determination

            Peter J. Judge, Garrick F. Taylor, Hugh R.W. Dannatt, and Anthony Watts

19. Native Mass Spectrometry: Towards High-Throughput Structural Proteomics

            Frances D.L. Kondrat, Weston B. Struwe, and Justin L.P. Benesch

Includes cutting-edge methods and protocols on structural proteomics

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras