Stem Cell Nanotechnology (2^nd Ed., 2nd ed. 2020) Methods and Protocols Methods in Molecular Biology Series, Vol. 2125

1. Design of a Versatile Sample Holder for Facile Culture of Cells on Electrospun Membranes or Thin Polymer Films under Flow Conditions

            Anne-Sophie Mertgen, Markus Rottmar, Lukas Weidenbacher, and Anne Géraldine Guex

2. Single Cell Densitometry and Weightlessness Culture of Mesenchymal Stem Cells Using Magnetic Levitation

3. Fluorescent Nanoclusters for Imaging of Cells/Stem Cells

            Fatemeh Molaabasi, Behnam Hajipour-Verdom, Mozhgan Alipour, and Seyed Morteza Naghib

4. Protocol for Determining the Induction of Human Embryonic Stem Cells into Myogenic Lineage Using Electrospun Nanofibers

            Hasham S. Sofi, Nisar Ahmad Khan, and Faheem A. Sheikh

5. Preparation and Characterization of Simvastatin Nanocapsules: Encapsulation of Hydrophobic Drugs in Calcium Alginate

            Mazaher Ahmadi, Tayyebeh Madrakian, and Saeid Ghavami

6. Multifunctional Magnetic Nanoparticles-Labeled Mesenchymal Stem Cells for Hyperthermia and Bioimaging Applications

            Sadegh Ghorbanzade, Seyed Morteza Naghib, Ali Sadr, Fatemeh Sadat Fateminia, Ali Ghaffarinejad, Keivan Majidzadeh-A, and Amir Sanati-Nezhad

7. Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNP) for Efficient Coupling of Biomolecules

            Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, Sivakumar Arumugam, and Selvamani Palaniswamy

8. Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells through Polymeric Nanoparticles

            Taha Umair Wani, Hasham S. Sofi, Nisar Ahmad Khan, and Faheem A. Sheikh

9. Methodology Involved in the Osteogenic Differentiation of Mesenchymal Stem Cells on Chitosan-Collagen Nanofibers Incorporated with Titanium Dioxide Nanoparticles

            Roqia Ashraf, Hasham S. Sofi, and Faheem A. Sheikh

10. Experimental Protocol for Culture and Differentiation of Osteoblasts on 3D Abode Using Nanofiber Scaffolds

            Hasham S. Sofi, Roqia Ashraf, and Faheem A. Sheikh

11. Experimental Protocol of MSCs Differentiation into Neural Lineage for Nerve Tissue Regeneration Using Polymeric Scaffolds

            Roqia Ashraf, Hasham S. Sofi, and Faheem A. Sheikh

12. Fabrication and Electrospinning of 3D-Biodegradable Poly-L-Lactic Acid (PLLA) Nanofibers for Clinical Application

            Balasundari Ramesh, Kotturathu Mammen Cherian, and Adegbenro Omotuyi John Fakoya

13. Differentiation of Menstrual Blood Stem Cells into Keratinocyte-Like Cells on Bilayer Nano-Fibrous Scaffold

            Shaghayegh Arasteh, Sara Katebifar, Reza Shirazi, and Somaieh Kazemnejad

14. Dental Pulp Stem Cells in Customized 3D Nanofibrous Scaffolds for Regeneration of Peripheral Nervous System

            Siddhartha Das and Jayesh R. Bellare

15. Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Endosome and Lysosome Isolation from Eukaryotic Cell

            Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, Dinh Toi Chu, and Sivakumar Arumugam

16. Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Plasma Membrane Isolation from Eukaryotic Cell

            Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, and Dinh Toi Chu

17. Nanoscaled Materials for Drug Delivery into Cells/Stem Cells

18. Method for Evaluating Neuromodulatory Properties of Dental Pulp Stem Cell as an In Vitro Model for Parkinson’s Disease

            Nareshwaran Gnanasegaran, Deepak B. Thimiri Govinda Raj, and Sivakumar Arumugam

19. Detection and Monitoring of Stem Cell Differentiation Using Nanotechnology

            Esfandyar Askari and Seyed Morteza Naghib

20. BacMam System for Rapid Recombinant Protein Expression in Mammalian Cells

            Deepak B. Thimiri Govinda Raj

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts