Sorting and Recycling Endosomes Methods in Cell Biology Series
Sorting and Recycling Endosomes provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane.
In recent years, the importance of endosomes as a sorting and recycling compartment has become increasingly appreciated. As such, scientists from various fields of cell biology, membrane traffic, and beyond, see the needs to communicate and learn about the methods used to investigate the dynamics and functions of endosomes.
This book brings together specialists from the field who contribute their expertise on a broad range of biomedical topics that will provide ideal reading for researchers interested in endosomal sorting and recycling. This volume covers the approaches necessary to study the key components that mediate the generation and transport of membrane-bounded carriers from the endosomes, and how membrane trafficking machinery is coordinated with cytoskeletons during these processes. In addition to studies carried out in mammalian cells, other model systems such as worm and yeast are also included.
Adam A. Wall, Nicholas D. Condon, Jeremy C. Yeo, Nicholas A. Hamilton and Jennifer L. Stow
2. Analyzing the Functions of Rab11-Effector Proteins During Cell Division
Rytis Prekeris
3. Expression and Localization of Exocytic and Recycling Rabs from Magnaportheoryzae in Mammalian Cells
Yaoyao Qi, M. Caleb Marlin, Zhimin Liang, Dongmei Zhang, Jie Zhou, Zonghua Wang, Guodong Lu and Guangpu Li
4. Methods to Analyze Novel Liaisons Between Endosomes and Centrosomes
Hui-Fang Hung, Heidi Hehnly and Stephen Doxsey
5. Biochemical Analysis of Rabin8, the Guanine Nucleotide Exchange Factor for Rab8
Bin Wu, Juanfei Wang, Yuting Zhao and Wei Guo
6. Simple In vitro Assay of Arf GAPs and Preparation of Arf Proteins as Substrates
Pei-Wen Chen, Xiaoying Jian, Ruibai Luo and Paul A. Randazzo
7. An ACAP1 Coat Complex Acting in Endocytic Recycling
Jian Li and Victor W. Hsu
8. Biochemical Methods for Studying Kinetic Regulation of Arf1 Activation by Sec7
Brian C. Richardson and J. Christopher Fromme
9. Rab and Arf G Proteins in Endosomal Trafficking
Dipannita Dutta and Julie G. Donaldson
10. Qualitative and Quantitative Analysis of Endocytic Recycling
James B. Reineke, Shuwei Xie, Naava Naslavsky and Steve Caplan
11. Methods for Analyzing the Role of Phospholipase A2 Enzymes in Endosome Membrane Tubule Formation
Danielle N. Kalkofen, Paul de Figueiredo and William J. Brown
12. In vivo Analysis of Recycling Endosomes in C. elegans
Anbing Shi and Barth D. Grant
13. Monitoring Receptor Trafficking Following Retromer and WASH Deregulation
Douglas G. Osborne, Christine A Phillips-Krawczak and Daniel D. Billadeau
14. Quantitative Analysis of Endosome Tubulation and Microdomain Organization Mediated by the WASH Complex
Emmanuel Derivery and Alexis Gautreau
15. Rab GTPases and Kinesin Motors in Endosomal Trafficking
Cédric Delevoye and Bruno Goud
16. Measuring Receptor Recycling in Polarized MDCK Cells
Luciana Gallo and Gerard Apodaca
17. Structural and Functional Analysis of Endosomal Compartments in Epithelial Cells
Andres E. Perez Bay, Ryan Schreiner and Enrique Rodriguez-Boulan
18. Analyzing the Role of AP-1B in Polarized Sorting from Recycling Endosomes in Epithelial Cells
Heike Fölsch
19. Recycling of the Insulin Responsive Glucose Transporter Glut4 Regulated by the Small GTPase RalA and the Exocyst Complex
Chao Nie and Xiao-Wei Chen
20. GPCR Sorting at the Multivesicular Endosomes
Michael Robert Dores and JoAnn Trejo
21. Visualizing and Quantitating Sequence-Dependent GPCR Recycling
Shanna L. Bowman, Amanda L. Soohoo and Manojkumar A. Puthenveedu
22. Methods to Study Endocytic Trafficking of the EGF Receptor
Itziar Pinilla-Macua and Alexander Sorkin
- Provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane.
- Covers an increasingly appreciated field in cell biology
- Includes both established and new technologies
- Brings together specialists from the field who contribute their expertise on a broad range of biomedical topics that will provide ideal reading for researchers interested in endosomal sorting and recycling
Date de parution : 09-2015
Ouvrage de 416 p.
19x23.3 cm
Thème de Sorting and Recycling Endosomes :
Mots-clés :
(PLA2; ) enzymes; Abscission; ACAP1; Actin; ADP-ribosylation factor; AP-1B; Appendages; Arf GAPs; Arf1; Arf6; Arp2/3; Basolateral membrane; Caenorhabditis elegans; Cargo sorting; Cenexin; Centriolin; Centrosome isolation; Centrosome; Clathrin; Clathrin-dependent internalization; Clathrin-independent endocytosis; Clathrin-independent internalization; Clathrin-mediated endocytosis; Coat protein; Coelomocytes; Cytokinesis; Degradation and recycling assay; Digital image analysis; EGF receptor; EHD1; Electron microscopy; Endocytic recycling; Endocytic tracers; Endocytosis; Endosomal recycling; Endosome dynamics; Endosome fission; Endosome tubules; Endosome; Endosomes; Enzyme assay; Epidermal growth factor; Epithelia; Epithelial cell; Epithelial cells; ERK1/2; Evi5; F-actin; Fip3; FIP3; Flow cytometry; G protein-coupled receptor (GPCR)Intraluminal vesicle; G protein-coupled receptor; G proteins; GEF; Glucose transporter; Glut4; GTPase-activating protein; Guanine nucleotide exchange factor (GEF)Sec7; Immunofluorescence; In vitro reconstitution; In vitro; Insulin signaling; Integrin; Iodination; Kinesin; Live cell imaging; LLC-PK1; Lysosomal degradation; Lysosome; Macrophage; Magnaporthe oryzae; MDCK cell; MDCK; MICAL-L1; Microdomain; Midbody; Mitosis; Mother centriole; Multivesicular endosome (MVE)Rab5; Multivesicular endosome; Oocyte; Phagocytosis; Phospholipase A2; Polarized sorting; Quantitative analysis; Rab GTPase; Rab GTPases; Rab1; Rab11; Rab22; Rab5; Rab8; Rabin8; RalA GTPase; Receptor recycling; Receptor trafficking; Recycling endosome; Recycling endosomes; Recycling Rab; Recycling; Retrograde recycling; Retromer; SNAREs; Sorting endosome; Spindle pole; Super-ecliptic pHluorin; The exocyst complex; Total internal reflection fluorescence microscopy; Transcytosis; Transferrin receptor; Transferrin trafficking; Transferrin; trans-Golgi network (TGN)Vesicular transport; Transmembrane protein; Ubiquitin; Vamp3; WASH; Yeast two-hybrid
