Selenoproteins, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1661
This volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Part I: Bioinformatic Tools for Selenoprotein Identification and Evolution
1. SECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins
Marco Mariotti
2. Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins
Didac Santesmasses, Marco Mariotti, and Roderic Guigó
3. SelGenAmic: An Algorithm for Selenoprotein Gene Assembly
Liang Jiang and Qiong Liu
Part II: Selenoprotein Biosynthesis
4. Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing
Bradley A. Carlson, Byeong Jae Lee, Petra A. Tsuji, Paul R. Copeland, Ulrich Schweizer, Vadim N. Gladyshe, and Dolph L. Hatfield
5. Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs
Eric M. Cockman and Donna M. Driscoll
6. Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis
Olga Kossinova, Alexey Malygin, Alain Krol, and Galina Karpova
7. In vitro Translation Assays for Selenocysteine Insertion
Mark H. Pinkerton and Paul R. Copeland
8. Studying Selenoprotein mRNA Translation using RNA-Seq and Ribosome Profiling
Brian K. Dalley, Lisa Baird, and Michael T. Howard
9. Modification of Selenoprotein mRNAs by Cap Tri-methylation
Anne-Sophie Gribling-Burrer, Gilbert Eriani, and Christine Allmang
Part III: Selenoprotein Detection and Quantification
10. Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
Véronique Vacchina and Jean Dumont
11. Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC – ICP MS)
Véronique Vacchina, Katarzyna Bierla, Joanna Szpunar, and Ryszard Lobinski
12. Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum
Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera, and José Luis Gómez-Ariza
13. Radioactive 75Se Labeling and Detection of Selenoproteins
Sun Hee Yim, Ryuta Tobe, Anton A. Turanov, and Bradley A. Carlson
14. Non-radioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines
Jordan Sonet, Sandra Mounicou,and Laurent Chavatte
15. Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips
Jordan Sonet, Sandra Mounicou, and Laurent Chavatte
16. Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
Elisa Castañeda Santa Cruz, J. Susanne Becker, J. Sabine Becker, and Alessandra Sussulini
Part IV: Selenoprotein Overexpression, Purification, and Activity
17. Overexpression of Recombinant Selenoproteins in E. coli
Qing Cheng and Elias S.J. Arnér
18. Preparation of Selenocysteine-containing Forms of Human SELENOK and SELENOS
Zhengqi Zhang, Jun Liu, and Sharon Rozovsky
19. Selenocysteine Mediated Expressed Protein Ligation of SELENOM
Jun Liu, Qingqing Chen and Sharon Rozovsky
20. Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity
Lionel Tarrago, Emmanuel Oheix, Zalán Péterfi, and Vadim N. Gladyshev
21. Selective Evaluation of Thioredoxin Reductase Enzymatic Activities
Elias S.J. Arnér
Part V: In Vivo Studies
22. Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk
Catherine Méplan
23. Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis
Erik Schoenmakers and Krishna Chatterjee
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key note and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Date de parution : 05-2018
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