RNA Tagging, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2166
Part I: In Situ Detection of RNA Molecules Using FISH Techniques
1. Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization
Thomas Dresselhaus and Andrea Bleckmann
2. Quantitative Fluorescence In SituHybridization Detection of Plant mRNAs with Single-Molecule Resolution
Kun Huang, Mona Batish, Chong Teng, Alex Harkess, Blake C. Meyers, and Jeffrey L. Caplan
3. Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells
Jingze J. Wu and Alexander F. Palazzo
4. Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single Molecule FISH and Immunofluorescence
Evelina Tutucci and Robert H. Singer
Part II: In Vivo Imaging of RNA Transport and Localization
5. Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More
Michael Ryckelynck
6. Visualization of Transiently Expressed mRNA in Plants Using MS2
Eduardo José Peña and Manfred Heinlein
7. New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells
Xavier Pichon, Marie-Cécile Robert, Edouard Bertrand, Robert H. Singer, and Evelina Tutucci
8. In Vivo Imaging of Mobile mRNAs in Plants Using MS2
Kai-Ren Luo, Nien-Chen Huang, and Tien-Shin Yu
9. RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi
David Burnett, Alexander Lichius, and Jens Tilsner
Part III: Imaging and Analysis of RNA Uptake and Transport between Cells
10. Utilizing Potato Virus X to Monitor RNA Movement
Zhiming Yu, Sung Ki Cho, Pengcheng Zhang, Yiguo Hong, and David J. Hannapel
11. A Protocol for Non-Biased Identification of RNAs Transferred between Heterologous Mammalian Cell Types Using RNA Tagging, Cell Sorting, and Sequencing
Sandipan Dasgupta and Jeffrey E. Gerst
12. Synthesizing Fluorescently Labeled dsRNAs and sRNAs to Visualize Fungal RNA Uptake
Rachael Hamby, Ming Wang, Lulu Qiao, and Hailing Jin
13. Labeling of dsRNA for Fungal Uptake Detection Analysis
Matteo Galli, Jafargholi Imani, and Karl-Heinz Kogel
Part IV: Identification and Analysis of RNA-Binding Proteins
14. Using RNA Affinity Purification Followed by Mass Spectrometry to Identify RNA-Binding Proteins (RBPs)
Mengge Shan and Brian D. Gregory
15. Plant Individual Nucleotide Resolution Crosslinking and Immunoprecipitation to Characterize RNA-Protein Complexes
Tino Köster and Dorothee Staiger
16. A Single-Molecule RNA Mobility Assay to Identify Proteins that Link RNAs to Molecular Motors
Varun Bhaskar, Min Jia, and Jeffrey A. Chao
17. Proximity-CLIP Provides a Snapshot of Protein-Occupied RNA Elements at Subcellular Resolution and Transcriptome-Wide Scale
Daniel Benhalevy and Markus Hafner
18. Double-Stranded RNA Pull-Down to Characterize Viral Replication Complexes in Plants
Marco Incarbone and Christophe Ritzenthaler
Part V: RNAs as Guides for Genome Editing and Imaging of Chromosome Loci
19. CRISPR Guide RNA Design Guidelines for Efficient Genome Editing
Patrick Schindele, Felix Wolter, and Holger Puchta
20. Live Cell CRISPR Imaging in Plant Cells with a Telomere-Specific Guide RNA
Solmaz Khosravi, Steven Dreissig, Patrick Schindele, Felix Wolter, Twan Rutten, Holger Puchta, and Andreas Houben
21. Live-Cell Imaging of Genomic Loci Using CRISPR/Molecular Beacon Hybrid Systems
Xiaotian Wu, Yachen Ying, Shiqi Mao, Christopher J. Krueger, and Antony K. Chen
22. Using RNA Tags for Multicolor Live Imaging of Chromatin Loci and Transcription in Drosophila Embryos
Hongtao Chen and Thomas Gregor
Part VI: Specific Techniques for the Analysis of RNA Functions
23. Integrated Genome-Scale Analysis and Northern Blot Detection of Retrotransposon siRNAs across Plant Species
Marcel Böhrer, Bart Rymen, Christophe Himber, Aude Gerbaud, David Pflieger, Debbie Laudencia-Chingcuanco, Amy Cartwright, John Vogel, Richard Sibout, and Todd Blevins
24. Transfection of Small Non-Coding RNAs into Arabidopsis thaliana Protoplasts
Stéphanie Lalande, Marjorie Chery, Elodie Ubrig, Guillaume Hummel, Julie Kubina, Angèle Geldreich, and Laurence Drouard
25. In Vivo Reporter System for miRNA-Mediated RNA Silencing
Károly Fátyol
26. TRAP-SEQ of Eukaryotic Translatomes Applied to the Detection of Polysome-Associated Long Non-Coding RNAs
Soledad Traubenik, Flavio Blanco, María Eugenia Zanetti, and Mauricio A. Reynoso
27. Posttranscriptional Suzuki-Miyaura Cross-Coupling Yields Labeled RNA for Conformational Analysis and Imaging
Manisha B. Walunj and Seergazhi G. Srivatsan
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 07-2021
Ouvrage de 490 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 116,04 €
Ajouter au panierDate de parution : 07-2020
Ouvrage de 490 p.
17.8x25.4 cm