Retinal Development, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2092

This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems.

1. Investigating Neurogenesis in Birds

Tania Rodrigues, Laurent Brodier, and Jean-Marc Matter

2. Studying in vivo Retinal Progenitor Cell Proliferation in Xenopus laevis

Cindy X. Kha, Dylan J. Guerin, and Kelly Ai-Sun Tseng

3. Three-dimensional Culture of Mouse Eyecups

Raven Diacou, Punita Bhansali, and Wei Liu

4. Live Imaging of Mouse Retinal Slices

Anthony P. Barrassoand Ross A. Poché

5. Retinal Strip Culture for Studying Ganglion Cell Axon Growth

6. Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants

Chuan-Chin Chiao, Chin-I Lin, and Meng-Jung Lee

7. Adeno-associate Virus as Gene Delivery Vehicle into Retina

Shuyun Deng and Kazuhiro Oka

8. Transposon-mediated Stable Suppression of Gene Expression in the Developing Chick Retina

Masaru Nakamoto and Chizu Nakamoto

9. A Simple Guide for Generating BAC Transgenic Animals for Retinal Research

Cavit Agca and Christian Grimm

10. Identification and Characterization of Cis-regulatory Elements for Photoreceptor Type-specific Transcription in Zebrafish

Wei Fang, Yi Wen, and Xiangyun Wei

11. Ultrasensitive RNAscope In Situ Hybridization System on Embryonic and Adult Mouse Retinas

12. Single Cell Capture, RNA-seq and Transcriptome Analysis from the Neural Retina

Rachayata Dharmat, Sangbae Kim, Yumei Li, and Rui Chen 

13. Genetically Directed Sparse Labeling System for Anatomical Studies of Retinal Ganglion Cells

Leila Jamal, Takae Kiyama, and Chai-An Mao

14. Intracellular Dye Microinjection in Retinal Morphological and Communication Studies

Ye Long

15. Multi-electrode Array Recording of Mouse Retinas Transplanted with Stem-cell Derived Retinal Sheets

Hung-Ya Tu and Take Matsuyama

Nange Jin, Zhijing Zhang, Kimberly A. Mankiewicz, and Christophe P. Ribelayga

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts