Proximity Labeling, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 2008
The EMARS Reaction for Proximity Labeling.- Proteomic Proximity Labelling to Reveal Interactions Between Biomolecules.- Establishing Cell Culture-based Experimental Setups for Proximity Labeling Using Ascorbate Peroxidase (APEX).- APEX Peroxidase Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.- Identification of Lipid Droplet Proteomes by Proximity Labeling Proteomics Using APEX2.- Antibody-driven Proximity Labeling in Fixed Tissues.- BioID: A Method to Generate a History of Protein Associations.- Direct Identification of Biotinylated Proteins from Proximity Labeling (Spot-BioID).- Identifying Synaptic Proteins by in vivo BioID from Mouse Brain.- A Proximity Ligation-based Method to Detect RNA-DNA Association.- Enzymatic Transfer of Photo-cross-linkers for RNA-Protein Photo-cross-linking at the mRNA 5'-cap.- Proximity-induced Bioorthogonal Chemistry Using Inverse Electron Demand Diels-Alder Reaction.- Overcoming the Limitations of Sortase with Proximity-based Sortase-mediated Ligation (PBSL).- Proximity-driven Site-specific and Covalent Labeling of Proteins with a TexasRed Fluorophore Reacting (ReacTR) Peptide Tag.- Affinity Conjugation for Rapid and Covalent Labeling of Proteins in Live Cells.- Ligand-directed N-Sulfonyl Pyridone Chemistry for Selective Native Protein Labeling and Imaging in Live Cell.
Date de parution : 08-2020
Ouvrage de 231 p.
17.8x25.4 cm
Date de parution : 05-2019
Ouvrage de 231 p.
17.8x25.4 cm