Protein Nanotechnology (3^rd Ed., 3rd ed. 2020) Protocols, Instrumentation, and Applications Methods in Molecular Biology Series, Vol. 2073

Preface…
Table of Contents…
Contributing Authors…

1. Introduction to Protein Nanotechnology
Laura J. Domigan and Juliet A. Gerrard

Part I Design and Production of Protein Building Blocks for Nanotechnology

2. Protein Expression in the Baculovirus-Insect Cell Expression System
Adetayo A. Adeniyi and Linda Hwee-Lin Lua

3. Peroxiredoxin Proteins as Building Blocks for Nanotechnology
Frankie Conroy and N. Amy Yewdall

4. Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins
Sarah R. Ball, Chi L. L. Pham, Victor Lo, Ann H. Kwan, and Margaret Sunde

5. ATP Synthase: Expression, Purification, and Function
Meghna Sobti, Robert Ishmukhametov, and Alastair G. Stewart

6. Molecular Superglues: Discovery and Engineering Orthogonalization
Paul Young and Christopher Squires

7. Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes
Samuel Lim, Douglas S. Clark, and Dominic J. Glover

Part II Towards Protein Nano-Devices

8. Adding Function to Protein Scaffolds
Kyle Webster, Luigi Sasso, and Laura J. Domigan

9. Virus-Derived Nanoparticles
Noor H. Dashti and Frank Sainsbury

10. Strategies for Increasing Protein Stability
Peter G. Chandler, Sebastian S. Broendum, Blake T. Riley, Matthew A. Spence, Colin J. Jackson, Sheena McGowan, and Ashley M. Buckle

11. Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol
Francesco Canfarotta, Sergey A. Piletsky, and Nicholas W. Turner

12. Nanotechnology with S-Layer Proteins
Bernhard Schuster and Uwe B. Sleytr

Part III Meaningful Measurements of Protein Nanocomponents

13. Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy
Lou Brillault and Michael J. Landsberg

14. Atomic Force Microscopy of Proteins
Yiran An, Sesha Sarathchandra Manuguri, and Jenny Malmstrom

15. Native Protein Mass Spectrometry
Timothy M. Allison and Mark T. Agasid

16. Nanoparticle Tracking Analysis of b-Casein Nanocarriers
Hikmat Masri and Jared K. Raynes

17.  Molecular Dynamics Simulation of Proteins
Thomas A. Collier, Thomas J. Piggot, and Jane R. Allison

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts