Protein Microarrays for Disease Analysis, 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2344

Editor Biographies…
Preface…
Table of Contents…
Contributing Authors…

Part I Introduction

1. Protein Microarrays Based Proteomics for Disease Analysis
Rodrigo Barderas, Sanjeeva Srivastava, and Joshua LaBaer

Part II Design and Production of Protein Microarrays

2. Systematic and Rational Design of Protein Arrays in Non-Contact Printers: Pipeline and Critical Aspects
Pablo Juanes-Velasco, Alicia Landeira-Viñuela, Angela-Patricia Hernandez, and Manuel Fuentes

3. Phage Microarrays for Screening of Humoral Immune Responses
Ana Montero-Calle, Pablo San Segundo-Acosta, María Garranzo-Asensio, Guillermo Solís-Fernández, Maricruz Sanchez-Martinez, and Rodrigo Barderas

4. Identification of Antibody Biomarker using High-Density Nucleic Acid Programmable Protein Array
Lusheng Song, Peter Wiktor, Ji Qiu, and Joshua LaBaer

5. Bead-Based Assays for Validating Proteomic Profiles in Body Fluids
Annika Bendes, Matilda Dale, Cecilia Mattsson, Tea Dodig-Crnković, Maria Jesus Iglesias, Jochen M. Schwenk, and Claudia Fredolini

Part III Applications of Protein Microarrays

6. Analysis of Protein-Protein Interactions by Protein Microarrays
Ana Montero-Calle and Rodrigo Barderas

7. Detection of Post-Translational Modification Autoantibodies using Peptide Microarray
Meng Li, Hongye Wang, Jiayu Dai, Meng Xu, Jianhua Liu, Jing Ren, Xiaosong Qin, Xianjiang Kang, and Xiaobo Yu

8. Epitope Mapping of Allergenic Lipid Transfer Proteins
Clara San Bartolomé, Carmen Oeo-Santos, Pablo San Segundo-Acosta, Rosa Muñoz-Cano, Javier Martínez-Botas, Joan Bartra, and Mariona Pascal

9. Epitope Mapping of Food Allergens Using Non-Contact Piezoelectric Microarray Printer
Javier Martínez-Botas, Carlos Fernández Lozano, Alberto Rodríguez Alonso, Laura Sánchez Ruano, and Belén de la Hoz

Part IV Deciphering Immune Responses in Diseases

10. Protein Arrays for the Identification of Seroreactive Protein Markers for Infectious Diseases
Apoorva Venkatesh, Aarti Jain, Huw Davies, Philip L. Felgner, Pradipsinh. K. Rathod, Swati Patankar, and Sanjeeva Srivastava

11. Glass Slide-Printed Protein Arrays as a Platform to Discover Serodiagnostic Antigens against Bacterial Infections
Alfonso Olaya-Abril and Manuel J. Rodríguez-Ortega

12. Affinity Proteomics Assays for Cardiovascular and Atherosclerotic Disease Biomarkers
Maria Jesus Iglesias, Jochen M. Schwenk, and Jacob Odeberg

13. Nucleic Acid Programmable Protein Arrays (NAPPA) for the Discovery of Autoantibodies in Osteoarthritis
Lucía Lourido, María Camacho-Encina, Francisco J. Blanco, and Cristina Ruiz-Romero

14. Profiling Autoantibody Responses to Devise Novel Diagnostic and Prognostic Markers using High-Density Protein Microarrays
Shabarni Gupta, Arghya Banerjee, Parvez Syed, and Sanjeeva Srivastava

Part V Antibody Microarrays for the Characterization of Disease Specific Protein Profiles 

15. Deciphering Intracellular Signaling Pathways in Tumoral Pathologies
Alicia Landeira-Viñuela, Pablo Juanes-Velasco, Rafael Gongora, Angela-Patricia Hernandez, and Manuel Fuentes

16. Combination of Antibody Arrays to Functionally Characterize Dark Proteins in Human Olfactory Neuroepithelial Cells
Mercedes Lachén-Montes, Karina Ausín, Paz Cartas-Cejudo, Joaquín Fernández-Irigoyen, and Enrique Santamaría

17. Protein Microarrays for Ocular Diseases
Guillermo Solís-Fernández, Ana Montero-Calle, Miren Alonso-Navarro, Miguel Ángel Fernandez-Torres, Victoria Eugenia Lledó, Maria Garranzo-Asensio, Rodrigo Barderas, and Ana Guzman-Aranguez

Part VI Statistical Methods for Analyzing Protein Microarray

18. Statistical Methods for Analysis of Protein Microarray Data using R
Yunro Chung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts