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Protein Acetylation, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1983

Langue : Anglais
Couverture de l’ouvrage Protein Acetylation

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.



Preface…
Table of Contents…
Contributing Authors…

Part I Detection, Quantification, and Isolation of Acetylated Protein Targets

1. High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets
Birgit Schilling, Jesse G. Meyer, Lei Wei, Melanie Ott, and Eric Verdin

2. Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry
Paula A. Agudelo Garcia, Miranda Gardner, Michael A. Freitas, and Mark R. Parthun

3. Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty
Pavlo Lazarchuk, Sunetra Roy, Katharina Schlacher, and Julia Sidorova

Part II Lysine Acetylation

4. Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC
Catherine W. Njeri, Onyekachi E. Ononye, and Lata Balakrishnan

5. Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo
Anahita Lashgari, Jean-Philippe Lambert, and Jacques Côté

6. Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments
Anastasia J. Lindahl, Alexis J. Lawton, Josue Baeza, James A. Dowell, and John M. Denu

7. Lysine Acetylation of Proteins and its Characterization in Human Systems
David K. Orren and Amrita Machwe

Part III Sirtuin Targets of Acetylation

8. Molecular and Cellular Characterization of SIRT1 Allosteric Activators
Michael B. Schultz, Conrad Rinaldi, Yuancheng Lu, João A. Amorim, and David A. Sinclair

9. Assessment of SIRT2 Inhibitors in Mouse Models of Cancer
Yashira L. Negrón Abril, Irma Fernández, and Robert S. Weiss

Part IV Modulation of DNA Repair by Acetylation

10. Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair 
Amelia J. Hodges, Steven A. Roberts, and John J. Wyrick

11. Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes
Shrabasti Roychoudhury, Suravi Pramanik, Hannah L. Harris, and Kishor K. Bhakat

12. Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation
Onyekachi E. Ononye, Catherine W. Njeri, and Lata Balakrishnan

13. Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons
Ping-Chieh Pao, Jay Penney, and Li-Huei Tsai

Part V DNA Interactive Proteins and Processing Enzymes Affected by Acetylation

14. Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability
Chenlin Song and Ingrid Grummt

15. Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling 
Marina Farkas and Steven B. McMahon

Part VI Influence of Acetylation Processes on Neuroepigenetics and Tumorigenesis

16. Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroepigenetics
Craig Myrum and Peter R. Rapp

17. Functional Analysis of HDACs in Tumorigeneis
Melissa Hadley, Satish Noonepalle, Debarati Banik, and Alejandro Villagra

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key note and implementation advice from the experts

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